Literature DB >> 25327141

Structure of the lysine specific protease Kgp from Porphyromonas gingivalis, a target for improved oral health.

Michael A Gorman1, Christine A Seers, Belinda J Michell, Susanne C Feil, N Laila Huq, Keith J Cross, Eric C Reynolds, Michael W Parker.   

Abstract

The oral pathogen Porphyromonas gingivalis is a keystone pathogen in the development of chronic periodontitis. Gingipains, the principle virulence factors of P. gingivalis are multidomain, cell-surface proteins containing a cysteine protease domain. The lysine specific gingipain, Kgp, is a critical virulence factor of P. gingivalis. We have determined the X-ray crystal structure of the lysine-specific protease domain of Kgp to 1.6 Å resolution. The structure provides insights into the mechanism of substrate specificity and catalysis.
© 2014 The Protein Society.

Entities:  

Keywords:  Kgp; Porphyromonas gingivalis; gingipains; keystone pathogen; lysine specific protease; periodontitis

Mesh：

Substances：

Year:  2014        PMID： 25327141      PMCID： PMC4282422          DOI： 10.1002/pro.2589

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  17 in total

1.  Experimental assessment of differences between related protein crystal structures.

Authors:  G J Kleywegt
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-11

2.  Kgp and RgpB, but not RgpA, are important for Porphyromonas gingivalis virulence in the murine periodontitis model.

Authors:  Rishi D Pathirana; Neil M O'Brien-Simpson; Gail C Brammar; Nada Slakeski; Eric C Reynolds
Journal:  Infect Immun       Date:  2007-01-12       Impact factor: 3.441

3.  Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features.

Authors:  W Kabsch; C Sander
Journal:  Biopolymers       Date:  1983-12       Impact factor: 2.505

4.  Crystal structure of gingipain R: an Arg-specific bacterial cysteine proteinase with a caspase-like fold.

Authors:  A Eichinger; H G Beisel; U Jacob; R Huber; F J Medrano; A Banbula; J Potempa; J Travis; W Bode
Journal:  EMBO J       Date:  1999-10-15       Impact factor: 11.598

5.  Flow cytometric analysis of adherence of Porphyromonas gingivalis to oral epithelial cells.

Authors:  Rishi D Pathirana; Neil M O'Brien-Simpson; Kumar Visvanathan; John A Hamilton; Eric C Reynolds
Journal:  Infect Immun       Date:  2007-03-05       Impact factor: 3.441

Review 6.  Molecular genetics and nomenclature of proteases of Porphyromonas gingivalis.

Authors:  M A Curtis; H K Kuramitsu; M Lantz; F L Macrina; K Nakayama; J Potempa; E C Reynolds; J Aduse-Opoku
Journal:  J Periodontal Res       Date:  1999-11       Impact factor: 4.419

7.  Integration, scaling, space-group assignment and post-refinement.

Authors:  Wolfgang Kabsch
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-01-22

8.  Porphyromonas gingivalis RgpA-Kgp proteinase-adhesin complexes penetrate gingival tissue and induce proinflammatory cytokines or apoptosis in a concentration-dependent manner.

Authors:  Neil M O'Brien-Simpson; Rishi D Pathirana; Glenn D Walker; Eric C Reynolds
Journal:  Infect Immun       Date:  2008-12-29       Impact factor: 3.441

Review 9.  The keystone-pathogen hypothesis.

Authors:  George Hajishengallis; Richard P Darveau; Michael A Curtis
Journal:  Nat Rev Microbiol       Date:  2012-09-03       Impact factor: 60.633

Review 10.  Scaling and assessment of data quality.

Authors:  Philip Evans
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14
View more
  7 in total

1.  Intermolecular latency regulates the essential C-terminal signal peptidase and sortase of the Porphyromonas gingivalis type-IX secretion system.

Authors:  Danuta Mizgalska; Theodoros Goulas; Arturo Rodríguez-Banqueri; Florian Veillard; Mariusz Madej; Ewelina Małecka; Katarzyna Szczesniak; Miroslaw Ksiazek; Magda Widziołek; Tibisay Guevara; Ulrich Eckhard; Maria Solà; Jan Potempa; F Xavier Gomis-Rüth
Journal:  Proc Natl Acad Sci U S A       Date:  2021-09-30       Impact factor: 11.205

2.  Structural and functional studies of legumain-mycocypin complexes revealed a competitive, exosite-regulated mode of interaction.

Authors:  Tasneem Elamin; Naiá P Santos; Peter Briza; Hans Brandstetter; Elfriede Dall
Journal:  J Biol Chem       Date:  2022-09-15       Impact factor: 5.486

3.  Porphyromonas gingivalis Uses Specific Domain Rearrangements and Allelic Exchange to Generate Diversity in Surface Virulence Factors.

Authors:  Stuart G Dashper; Helen L Mitchell; Christine A Seers; Simon L Gladman; Torsten Seemann; Dieter M Bulach; P Scott Chandry; Keith J Cross; Steven M Cleal; Eric C Reynolds
Journal:  Front Microbiol       Date:  2017-01-26       Impact factor: 5.640

4.  Existence of natural mouse IgG mAbs recognising epitopes shared by malondialdehyde acetaldehyde adducts and Porphyromonas gingivalis.

Authors:  Mikael Kyrklund; Heidi Kaski; Ramin Akhi; Antti E Nissinen; Outi Kummu; Ulrich Bergmann; Pirkko Pussinen; Sohvi Hörkkö; Chunguang Wang
Journal:  Innate Immun       Date:  2021-01-14       Impact factor: 2.680

5.  Crystal Structure and Activity Studies of the C11 Cysteine Peptidase from Parabacteroides merdae in the Human Gut Microbiome.

Authors:  Karen McLuskey; Jaspreet S Grewal; Debanu Das; Adam Godzik; Scott A Lesley; Ashley M Deacon; Graham H Coombs; Marc-André Elsliger; Ian A Wilson; Jeremy C Mottram
Journal:  J Biol Chem       Date:  2016-03-03       Impact factor: 5.157

Review 6.  Pathogenesis of Important Virulence Factors of Porphyromonas gingivalis via Toll-Like Receptors.

Authors:  Lu Jia; Nannan Han; Juan Du; Lijia Guo; Zhenhua Luo; Yi Liu
Journal:  Front Cell Infect Microbiol       Date:  2019-07-18       Impact factor: 5.293

7.  Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity.

Authors:  Mohamed Azarkan; Erik Maquoi; François Delbrassine; Raphael Herman; Nasiha M'Rabet; Rafaèle Calvo Esposito; Paulette Charlier; Frédéric Kerff
Journal:  Sci Rep       Date:  2020-11-11       Impact factor: 4.379
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.