Literature DB >> 25323713

Genome Sequence of an Extremely Drug-Resistant Clinical Isolate of Acinetobacter baumannii Strain AB030.

Peter C Loewen¹, Yasser Alsaadi¹, Dinesh Fernando¹, Ayush Kumar².

Abstract

We report the 4.3-Mbp genome sequence of a blood isolate of Acinetobacter baumannii strain AB030.

Entities: Chemical Disease Species

Year: 2014 PMID： 25323713 PMCID： PMC4200151 DOI： 10.1128/genomeA.01035-14

Source DB: PubMed Journal: Genome Announc

GENOME ANNOUNCEMENT

Acinetobacter baumannii is notorious for causing infections that are very difficult to treat because of its high resistance to all classes of antibiotics (1, 2). Multidrug-resistant (MDR), extremely drug-resistant (XDR), and pandrug-resistant (PDR) isolates have been reported from around the globe (3–7). Here, we report the complete genome sequence of A. baumannii strain AB030, an XDR isolate from a bloodstream infection in a 29-year-old female (8). A. baumannii strain AB030 is resistant to aminoglycosides (amikacin, gentamicin); carbapenems (imipenem, meropenem); fluoroquinolones (ciprofloxacin, levofloxacin, moxifloxacin); penicillin and β-lactamase inhibitors (tazobactam-piperacillin); first-generation (cefzolin) and extended-spectrum (cefepime, ceftriaxone) cephalosporins; tigecycline; and trimethoprim-sulfamethoxazole (8). It is susceptible only to colistin (8). Therefore, we classified this isolate as XDR following the recommendations made by Magiorakos et al. (9). DNA was isolated using the Ultra-Clean Microbial DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) following the manufacturer’s instructions. Genome sequencing was performed using the PacBio platform at the Genome Quebec facility (Montreal, QC, Canada) using three SMRT cells. Assembly was carried out using the PacBio SMRT analysis pipeline version 2.2.0, with 93.3× coverage to give a single contiguous genome sequence. The sequence was annotated by the National Center for Biotechnology Information (NCBI) Prokaryotic Genomes Annotation Pipeline. The genome consists of 4,335,793 bases with a G+C content of 39%. There are a total of 4,258 putative genes, which include 4,132 protein-, 18 rRNA-, and 73 tRNA-coding sequences.

Nucleotide sequence accession number.

The genome sequence of A. baumannii AB030 was deposited in NCBI GenBank under the accession number CP009257.

9 in total

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Authors: A-P Magiorakos; A Srinivasan; R B Carey; Y Carmeli; M E Falagas; C G Giske; S Harbarth; J F Hindler; G Kahlmeter; B Olsson-Liljequist; D L Paterson; L B Rice; J Stelling; M J Struelens; A Vatopoulos; J T Weber; D L Monnet
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Authors:
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