| Literature DB >> 25319892 |
Mathieu J F Crupi1, Douglas S Richardson, Lois M Mulligan.
Abstract
Cell surface biotinylation is a biochemical approach to covalently bind membrane-impermeable biotin to the extracellular domain of membrane proteins, such as receptor tyrosine kinases (RTKs). Subsequent to ligand incubation periods, activated biotinylated receptors may internalize from the cell surface into early endosomes and then travel through intracellular compartments to either recycle back to the membrane or degrade in lysosomes. The biotin-labeled proteins may be detected through affinity purification with streptavidin agarose resins. This chapter describes methods for cell surface biotinylation to assess RTK trafficking steps.Entities:
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Year: 2015 PMID： 25319892 DOI： 10.1007/978-1-4939-1789-1_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745