Armorel Diane van Eyk1. 1. Division of Pharmacology, Department of Pharmacy and Pharmacology, University of the Witwatersrand , Parktown , South Africa.
Abstract
CONTEXT: Artificial sweeteners (AS) have been associated with tumor development (including colon cancer) in both animals and humans although evidence has been conflicting. OBJECTIVES: Additional research was thus conducted by studying the effects of 5 AS on the morphology, cell proliferation and DNA in cells by utilizing Caco-2, HT-29 (colon) and HEK-293 (kidney) cell lines. MATERIALS AND METHODS: Cells were exposed to sodium cyclamate, sodium saccharin, sucralose and acesulfame-K (0-50 mM) and aspartame (0-35 mM) over 24, 48 and 72 hours. Morphological changes were presented photographically and % cell viability was determined by using the MTT cell viability assay. Possible DNA damage (comet assay) induced by the AS (0.1, 1 and 10 mM, treated for 24, 48 and 72 hours) was studied. The appearance of "comets" was scored from no damage to severe damage (0-4). RESULTS: Cells became flatter and less well defined at higher AS concentrations (>10 mM). At concentrations >10 mM, decreased cell viability was noted with both increasing concentration and increasing incubation time for all cell lines tested. In general, HEK-293 cells seemed to be less affected then the colon cancer cells. Sucralose and sodium saccharin seemed to elicit the greatest degree of DNA fragmentation of all the sweeteners tested in all the cell lines used. DISCUSSION: Morphological cell alterations, cell viability and DNA fragmentation seemed to be more in the colon cancer cells. CONCLUSIONS: Further studies have to be performed to clarify mechanisms involved causing these alterations in mammalian cells.
CONTEXT: Artificial sweeteners (AS) have been associated with tumor development (including colon cancer) in both animals and humans although evidence has been conflicting. OBJECTIVES: Additional research was thus conducted by studying the effects of 5 AS on the morphology, cell proliferation and DNA in cells by utilizing Caco-2, HT-29 (colon) and HEK-293 (kidney) cell lines. MATERIALS AND METHODS: Cells were exposed to sodium cyclamate, sodium saccharin, sucralose and acesulfame-K (0-50 mM) and aspartame (0-35 mM) over 24, 48 and 72 hours. Morphological changes were presented photographically and % cell viability was determined by using the MTT cell viability assay. Possible DNA damage (comet assay) induced by the AS (0.1, 1 and 10 mM, treated for 24, 48 and 72 hours) was studied. The appearance of "comets" was scored from no damage to severe damage (0-4). RESULTS: Cells became flatter and less well defined at higher AS concentrations (>10 mM). At concentrations >10 mM, decreased cell viability was noted with both increasing concentration and increasing incubation time for all cell lines tested. In general, HEK-293 cells seemed to be less affected then the colon cancer cells. Sucralose and sodium saccharin seemed to elicit the greatest degree of DNA fragmentation of all the sweeteners tested in all the cell lines used. DISCUSSION: Morphological cell alterations, cell viability and DNA fragmentation seemed to be more in the colon cancer cells. CONCLUSIONS: Further studies have to be performed to clarify mechanisms involved causing these alterations in mammalian cells.
Authors: Colin Berry; David Brusick; Samuel M Cohen; Jerry F Hardisty; V Lee Grotz; Gary M Williams Journal: Nutr Cancer Date: 2016-09-21 Impact factor: 2.900
Authors: Un Chul Shin; Jeong Su Choi; Yeon Jae Beak; Min Woo Lee; Hyung Soo Kim; Dal Woong Choi; Dong Gil Kim; Suhng Wook Kim Journal: J Labelled Comp Radiopharm Date: 2020-11-09 Impact factor: 1.921