Literature DB >> 25313201

Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.

Luis Gerardo Villa-Diaz1, Jin Koo Kim1, Joerg Lahann1, Paul H Krebsbach2.   

Abstract

We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous in vitro culture on PMEDSAH-grafted plates. Together, these data demonstrate the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine. ©AlphaMed Press.

Entities:  

Keywords:  Differentiation; Feeder-free culture; Induced pluripotent stem cells; Regenerative medicine; Stem cells; Synthetic coating; iPSC

Mesh：

Substances：

Year:  2014        PMID： 25313201      PMCID： PMC4250210          DOI： 10.5966/sctm.2014-0087

Source DB:  PubMed          Journal:  Stem Cells Transl Med        ISSN： 2157-6564            Impact factor:   6.940


  27 in total

1.  Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.

Authors:  Shuyuan Yao; Shuibing Chen; Julie Clark; Ergeng Hao; Gillian M Beattie; Alberto Hayek; Sheng Ding
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-21       Impact factor: 11.205

Review 2.  The development of 'feeder' cells for the preparation of clinical grade hES cell lines: challenges and solutions.

Authors:  Glyn N Stacey; Fernando Cobo; Ana Nieto; Paloma Talavera; Lyn Healy; Angel Concha
Journal:  J Biotechnol       Date:  2006-05-11       Impact factor: 3.307

3.  Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation.

Authors:  Paromita Deb-Rinker; Dao Ly; Anna Jezierski; Marianna Sikorska; P Roy Walker
Journal:  J Biol Chem       Date:  2004-12-21       Impact factor: 5.157

4.  A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells.

Authors:  Hong Fang Lu; Chou Chai; Tze Chiun Lim; Meng Fatt Leong; Jia Kai Lim; Shujun Gao; Kah Leong Lim; Andrew C A Wan
Journal:  Biomaterials       Date:  2014-01-09       Impact factor: 12.479

5.  Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.

Authors:  Katherine Amps; Peter W Andrews; George Anyfantis; Lyle Armstrong; Stuart Avery; Hossein Baharvand; Julie Baker; Duncan Baker; Maria B Munoz; Stephen Beil; Nissim Benvenisty; Dalit Ben-Yosef; Juan-Carlos Biancotti; Alexis Bosman; Romulo Martin Brena; Daniel Brison; Gunilla Caisander; María V Camarasa; Jieming Chen; Eric Chiao; Young Min Choi; Andre B H Choo; Daniel Collins; Alan Colman; Jeremy M Crook; George Q Daley; Anne Dalton; Paul A De Sousa; Chris Denning; Janet Downie; Petr Dvorak; Karen D Montgomery; Anis Feki; Angela Ford; Victoria Fox; Ana M Fraga; Tzvia Frumkin; Lin Ge; Paul J Gokhale; Tamar Golan-Lev; Hamid Gourabi; Michal Gropp; Guangxiu Lu; Ales Hampl; Katie Harron; Lyn Healy; Wishva Herath; Frida Holm; Outi Hovatta; Johan Hyllner; Maneesha S Inamdar; Astrid Kresentia Irwanto; Tetsuya Ishii; Marisa Jaconi; Ying Jin; Susan Kimber; Sergey Kiselev; Barbara B Knowles; Oded Kopper; Valeri Kukharenko; Anver Kuliev; Maria A Lagarkova; Peter W Laird; Majlinda Lako; Andrew L Laslett; Neta Lavon; Dong Ryul Lee; Jeoung Eun Lee; Chunliang Li; Linda S Lim; Tenneille E Ludwig; Yu Ma; Edna Maltby; Ileana Mateizel; Yoav Mayshar; Maria Mileikovsky; Stephen L Minger; Takamichi Miyazaki; Shin Yong Moon; Harry Moore; Christine Mummery; Andras Nagy; Norio Nakatsuji; Kavita Narwani; Steve K W Oh; Sun Kyung Oh; Cia Olson; Timo Otonkoski; Fei Pan; In-Hyun Park; Steve Pells; Martin F Pera; Lygia V Pereira; Ouyang Qi; Grace Selva Raj; Benjamin Reubinoff; Alan Robins; Paul Robson; Janet Rossant; Ghasem H Salekdeh; Thomas C Schulz; Karen Sermon; Jameelah Sheik Mohamed; Hui Shen; Eric Sherrer; Kuldip Sidhu; Shirani Sivarajah; Heli Skottman; Claudia Spits; Glyn N Stacey; Raimund Strehl; Nick Strelchenko; Hirofumi Suemori; Bowen Sun; Riitta Suuronen; Kazutoshi Takahashi; Timo Tuuri; Parvathy Venu; Yuri Verlinsky; Dorien Ward-van Oostwaard; Daniel J Weisenberger; Yue Wu; Shinya Yamanaka; Lorraine Young; Qi Zhou
Journal:  Nat Biotechnol       Date:  2011-11-27       Impact factor: 54.908

6.  Human embryonic stem cells express an immunogenic nonhuman sialic acid.

Authors:  Maria J Martin; Alysson Muotri; Fred Gage; Ajit Varki
Journal:  Nat Med       Date:  2005-01-30       Impact factor: 53.440

7.  Derivation of mesenchymal stem cells from human induced pluripotent stem cells cultured on synthetic substrates.

Authors:  L G Villa-Diaz; S E Brown; Y Liu; A M Ross; J Lahann; J M Parent; P H Krebsbach
Journal:  Stem Cells       Date:  2012-06       Impact factor: 6.277

8.  A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression.

Authors:  H O Li; Y F Zhu; M Asakawa; H Kuma; T Hirata; Y Ueda; Y S Lee; M Fukumura; A Iida; A Kato; Y Nagai; M Hasegawa
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

Review 9.  Concise review: The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings.

Authors:  L G Villa-Diaz; A M Ross; J Lahann; P H Krebsbach
Journal:  Stem Cells       Date:  2013-01       Impact factor: 6.277

10.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.

Authors:  Masato Nakagawa; Yukimasa Taniguchi; Sho Senda; Nanako Takizawa; Tomoko Ichisaka; Kanako Asano; Asuka Morizane; Daisuke Doi; Jun Takahashi; Masatoshi Nishizawa; Yoshinori Yoshida; Taro Toyoda; Kenji Osafune; Kiyotoshi Sekiguchi; Shinya Yamanaka
Journal:  Sci Rep       Date:  2014-01-08       Impact factor: 4.379
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  2 in total

1.  Engineered Human Stem Cell Microenvironments.

Authors:  Jacob H Jordahl; Luis Villa-Diaz; Paul H Krebsbach; Joerg Lahann
Journal:  Curr Stem Cell Rep       Date:  2016-02-03

2.  Nanotopography regulates motor neuron differentiation of human pluripotent stem cells.

Authors:  Weiqiang Chen; Shuo Han; Weiyi Qian; Shinuo Weng; Haiou Yang; Yubing Sun; Luis G Villa-Diaz; Paul H Krebsbach; Jianping Fu
Journal:  Nanoscale       Date:  2018-02-15       Impact factor: 7.790
  2 in total

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