Flavobacterium maotaiense sp. nov., isolated from freshwater.

Two novel strains, T9(T) and T10, were isolated from water samples collected from Chishui River flowing through Maotai town, Guizhou, south-west China. The isolates were yellow-pigmented, Gram-reaction-negative, rod-shaped, non-motile and aerobic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Flavobacterium, and showed highest similarities to Flavobacterium hibernum DSM 12611(T) (97.0 %), followed by Flavobacterium granuli Kw05(T) (96.7 %) and Flavobacterium pectinovorum DSM 6368(T) (96.7 %). The novel strains were able to grow at 20-37 °C (optimum 25 °C), pH 7.0-10.0 (optimum pH 7.0-8.0) and with 0-0.5 % (w/v) NaCl (optimum 0.5 %). The predominant fatty acids were iso-C15 : 0, C16 : 1ω7c, anteiso-C15 : 0, C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 1ω10c, and menaquinone-6 (MK-6) was the main respiratory quinone. The major polar lipids were phosphatidylethanolamine, one unknown glycolipid, two unknown aminolipids and two unidentified lipids. The DNA G+C contents of strains T9(T) and T10 were 37.7 and 36.4 mol%, respectively. According to the phenotypic and genetic data, strains T9(T) and T10 represent a novel species in the genus Flavobacterium, for which the name Flavobacterium maotaiense sp. nov. is proposed. The type strain is T9(T) ( = CGMCC 1.12712(T) = JCM 19927(T)).