Thérèse Samdapawindé Kagoné1, Cyrille Bisseye2, Nicolas Méda3, Jean Testa3, Virginio Pietra4, Dramane Kania3, Albert Théophane Yonli5, Tegwindé Rebeca Compaoré5, Jean Baptiste Nikiéma5, Comlan de Souza6, Jacques Simpore5. 1. Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso; Centre MURAZ Bobo-Dioulasso, Burkina Faso. 2. Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso; Laboratory of Molecular and Cellular Biology, University of Science and Technique of Masuku (USTM), Franceville, Gabon. Electronic address: cbisseye@gmail.com. 3. Centre MURAZ Bobo-Dioulasso, Burkina Faso. 4. Health Centre Saint Camille of Nanoro, Burkina Faso. 5. Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso. 6. Faculty of Sciences, University of Lomé, Togo.
Abstract
OBJECTIVE: To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5Δ32). METHODS: Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLA-B57*01 and CCR5Δ32 alleles were genotyped in 51 serodiscordant couples in Burkina Faso. DC-SIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, Italy). CCR5Δ32 deletion was investigated by PCR. RESULTS: The two SNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGN rs2287886 and HIV-1 remained significant (P<0.01). No association was found between IRF1 SNPs and HIV-1 infection. CCR5Δ32 wild type allele was found in 100% of serodiscordant couples. A high frequency of HLA-B57*01 allele was found in the HIV-positive (78%) compared with HIV-negative group (51%), however this difference was no longer significant after the correction of the sex confounding effect in the logistic regression model. CONCLUSIONS: Our study suggests a protective role of a variation of DC-SIGN promoter and genetic resistance to HIV-1 in serodiscordant couples in Burkina Faso.
OBJECTIVE: To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5Δ32). METHODS: Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLA-B57*01 and CCR5Δ32 alleles were genotyped in 51 serodiscordant couples in Burkina Faso. DC-SIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, Italy). CCR5Δ32 deletion was investigated by PCR. RESULTS: The two SNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGNrs2287886 and HIV-1 remained significant (P<0.01). No association was found between IRF1 SNPs and HIV-1 infection. CCR5Δ32 wild type allele was found in 100% of serodiscordant couples. A high frequency of HLA-B57*01 allele was found in the HIV-positive (78%) compared with HIV-negative group (51%), however this difference was no longer significant after the correction of the sex confounding effect in the logistic regression model. CONCLUSIONS: Our study suggests a protective role of a variation of DC-SIGN promoter and genetic resistance to HIV-1 in serodiscordant couples in Burkina Faso.
Authors: Gaby S Steba; Sylvie M Koekkoek; Joost W Vanhommerig; Kees Brinkman; David Kwa; Jan T M Van Der Meer; Maria Prins; Ben Berkhout; Michael Tanck; William A Paxton; Richard Molenkamp; Janke Schinkel Journal: J Infect Dis Date: 2018-01-17 Impact factor: 5.226