Sivaprabha Jambunathan1, Dharani Bangarusamy2, Palghat Raghunathan Padma2, Sumathi Sundaravadivelu3. 1. Department of Biotechnology, K.S. Rangasamy college of Arts and Science, Tiruchengode, Tamil Nadu, India. 2. Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore 641 043, Tamil Nadu, India. 3. Department of Biotechnology, K.S. Rangasamy college of Arts and Science, Tiruchengode, Tamil Nadu, India. Electronic address: sumathi_vnktsh@yahoo.co.in.
Abstract
OBJECTIVES: To evaluate the methanol extract of both the leaves and the rhizomes of Curcuma amada (C. amada) for their cytotoxic activity against breast cancer cell lines MCF-7 and MDA MB 231. METHODS: Viability and cytotoxicity induced by the extracts were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, sulforhodamine B, and lactate dehydrogenase release assays. Various staining techniques such as acridine orange/ethidium bromide, Giemsa, ethidium bromide, propidium iodide, and Hoechst 33342 staining were employed to study the mechanism of cell death induced by the extract. RESULTS: The results indicated that the methanol extract of both the leaves and the rhizomes of C. amada exhibited strong cytotoxicity towards breast cancer cell lines MCF-7 and MDA MB 231. The extract also showed less cytotoxicity towards non-cancerous breast cell line HBL-100. The results of staining revealed that the extracts induced cell death in cancer cells which are mediated through apoptotic pathway. CONCLUSIONS: The results indicated that the methanol extract of leaves and rhizomes of C. amada possess anticancer and cytotoxic activity.
OBJECTIVES: To evaluate the methanol extract of both the leaves and the rhizomes of Curcuma amada (C. amada) for their cytotoxic activity against breast cancer cell lines MCF-7 and MDA MB 231. METHODS: Viability and cytotoxicity induced by the extracts were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, sulforhodamine B, and lactate dehydrogenase release assays. Various staining techniques such as acridine orange/ethidium bromide, Giemsa, ethidium bromide, propidium iodide, and Hoechst 33342 staining were employed to study the mechanism of cell death induced by the extract. RESULTS: The results indicated that the methanol extract of both the leaves and the rhizomes of C. amada exhibited strong cytotoxicity towards breast cancer cell lines MCF-7 and MDA MB 231. The extract also showed less cytotoxicity towards non-cancerous breast cell line HBL-100. The results of staining revealed that the extracts induced cell death in cancer cells which are mediated through apoptotic pathway. CONCLUSIONS: The results indicated that the methanol extract of leaves and rhizomes of C. amada possess anticancer and cytotoxic activity.