Manila Yadav1, Amita Yadav1, Jaya Parkash Yadav2. 1. Department of Genetics, M.D. University, Rohtak-124001, Haryana, India. 2. Department of Genetics, M.D. University, Rohtak-124001, Haryana, India. Electronic address: yadav1964@rediffmail.com.
Abstract
OBJECTIVE: To elucidate the antioxidant activity and total phenolic content (TPC) of ethyl acetate extracts of endophytic fungi isolated from Eugenia jambolana by three different antioxidant assays. METHODS: Twenty one different endophytic fungal extracts were screened for presence of various phytochemicals, TPC and in vitro antioxidant activity. TPC was tested by Folin-Ciocalteau reagent based assay. DPPH free radical scavenging, hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidant activity. RESULTS: Alkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all 21 endophytes. A significant positive correlation was found between antioxidant activity and TPC in fungal extracts. There is 36% endophytic extracts having high phenolic content exhibited potent antioxidant activity. Chaetomium sp., Aspergillus sp., Aspergillus peyronelii and Aspergillus niger strain showed the highest antioxidant activity ranging from 50% to 80% having 58 mg/g to 60 mg/g GAE total phenolics. Ascorbic acid used as a standard showed 90% reducing potential. CONCLUSIONS: The results reveal that metabolites produced by endophytic fungi isolated from Eugenia jambolana can be a potential source of novel natural antioxidant compounds.
OBJECTIVE: To elucidate the antioxidant activity and total phenolic content (TPC) of ethyl acetate extracts of endophytic fungi isolated from Eugenia jambolana by three different antioxidant assays. METHODS: Twenty one different endophytic fungal extracts were screened for presence of various phytochemicals, TPC and in vitro antioxidant activity. TPC was tested by Folin-Ciocalteau reagent based assay. DPPH free radical scavenging, hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidant activity. RESULTS:Alkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all 21 endophytes. A significant positive correlation was found between antioxidant activity and TPC in fungal extracts. There is 36% endophytic extracts having high phenolic content exhibited potent antioxidant activity. Chaetomium sp., Aspergillus sp., Aspergillus peyronelii and Aspergillus niger strain showed the highest antioxidant activity ranging from 50% to 80% having 58 mg/g to 60 mg/g GAE total phenolics. Ascorbic acid used as a standard showed 90% reducing potential. CONCLUSIONS: The results reveal that metabolites produced by endophytic fungi isolated from Eugenia jambolana can be a potential source of novel natural antioxidant compounds.
Authors: Naveed Munir; Zahed Mahmood; Muhammad Shahid; Muhammad Naveed Afzal; Muhammad Jahangir; Syed Muhammad Ali Shah; Imtiaz Mahmood Tahir; Muhammad Riaz; Shoukat Hussain; Muhammad Akram; Fatima Yousaf Journal: Dose Response Date: 2022-03-01 Impact factor: 2.658
Authors: Nelson H Caicedo; Andres F Davalos; Paula A Puente; Ana Y Rodríguez; Paola A Caicedo Journal: Microbiologyopen Date: 2019-07-12 Impact factor: 3.139