Rong Hua1, Yongmei Zhang2, Fuxing Chen3, Zhonghai Zhou3, Xi Li4, Boming Shao2, Shangjing Wang5, Yujing Zhang4, Xiaoting Lv3. 1. Department of Emergency Medicine, The 97th Hospital of PLA, Xuzhou 221000, China. Electronic address: HuarongSCI@163.com. 2. Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221000, China. 3. Department of Central Laboratory, The 97th Hospital of PLA, Xuzhou 221000, China. 4. Department of Trauma Central, The 97th Hospital of PLA, Xuzhou 221000, China. 5. Department of Emergency Medicine, The 97th Hospital of PLA, Xuzhou 221000, China.
Abstract
OBJECTIVE: Posttraumatic immune disorder can cause complications including systemic inflammatory response syndrome (SIRS) and multiple-organ dysfunction syndrome (MODS). Cytotoxic granules containing perforin and granzyme-B (GrB) are released by cytotoxic CD8(+) T lymphocytes, NK and γδT cells after major trauma. This prospective clinical study was designed to analyze the association between these immune components and complications after major trauma. METHODS: We retrospectively studied 48 patients aged between 16 and 65 years admitted within 90min of major trauma (Injury Severity Score>16) and surviving beyond 7 days, and 20 healthy controls. Blood samples were drawn on admission and after 1, 3 and 7 days. CD8(+) T, NK and γδT cell counts in peripheral blood and the levels of perforin and GrB in these cells were analyzed by flow cytometry. Clinical aspects of MODS and SIRS were recorded daily. RESULTS: CD8(+) T cell counts were not significantly different in patients with SIRS or uncomplicated group, but were depressed in the MODS group after trauma. However, NK cell counts in patients with MODS were significantly depressed only at day 7 after injury, and γδT cell counts were significantly depressed after trauma. Perforin levels in CD8(+) T, NK and γδT cells in patients with MODS were depressed after trauma. GrB levels in NK, CD8(+) T and γδT cells in patients with MODS were significantly depressed at 3 and 7 days post trauma. CONCLUSION: Posttraumatic MODS is associated with early, sustained, and severe depression of lymphocytes.
OBJECTIVE:Posttraumatic immune disorder can cause complications including systemic inflammatory response syndrome (SIRS) and multiple-organ dysfunction syndrome (MODS). Cytotoxic granules containing perforin and granzyme-B (GrB) are released by cytotoxicCD8(+) T lymphocytes, NK and γδT cells after major trauma. This prospective clinical study was designed to analyze the association between these immune components and complications after major trauma. METHODS: We retrospectively studied 48 patients aged between 16 and 65 years admitted within 90min of major trauma (Injury Severity Score>16) and surviving beyond 7 days, and 20 healthy controls. Blood samples were drawn on admission and after 1, 3 and 7 days. CD8(+) T, NK and γδT cell counts in peripheral blood and the levels of perforin and GrB in these cells were analyzed by flow cytometry. Clinical aspects of MODS and SIRS were recorded daily. RESULTS:CD8(+) T cell counts were not significantly different in patients with SIRS or uncomplicated group, but were depressed in the MODS group after trauma. However, NK cell counts in patients with MODS were significantly depressed only at day 7 after injury, and γδT cell counts were significantly depressed after trauma. Perforin levels in CD8(+) T, NK and γδT cells in patients with MODS were depressed after trauma. GrB levels in NK, CD8(+) T and γδT cells in patients with MODS were significantly depressed at 3 and 7 days post trauma. CONCLUSION:Posttraumatic MODS is associated with early, sustained, and severe depression of lymphocytes.
Authors: Tomas Tyll; Pavlina Lyskova; Vit Hubka; Martin Muller; Lubomir Zelenka; Martina Curdova; Inna Tuckova; Miroslav Kolarik; Petr Hamal Journal: Mycopathologia Date: 2015-09-12 Impact factor: 2.574
Authors: Claudia P Cabrera; Joanna Manson; Joanna M Shepherd; Hew D Torrance; David Watson; M Paula Longhi; Mimoza Hoti; Minal B Patel; Michael O'Dwyer; Sussan Nourshargh; Daniel J Pennington; Michael R Barnes; Karim Brohi Journal: PLoS Med Date: 2017-07-17 Impact factor: 11.069