Literature DB >> 2530088

Vascular anticoagulant beta: a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning, expression and comparison with VAC-alpha.

R Hauptmann1, I Maurer-Fogy, E Krystek, G Bodo, H Andree, C P Reutelingsperger.   

Abstract

A cDNA was cloned coding for a new member of the human Ca2+-modulated phospholipid-binding protein family termed annexins. Due to its 56% identity to the human vascular anticoagulant (VAC) the new protein is named VAC-beta, renaming the previous VAC as VAC-alpha. Northern analysis detects one hybridizing mRNA species of 2.2 kb in human placenta. Genomic Southern blot analysis shows a VAC-beta gene of comparable complexity to the VAC-alpha gene. The cDNA was expressed in Escherichia coli and the recombinant protein purified to homogeneity. Antiserum raised against VAC-beta weakly cross-reacts with VAC-alpha. The properties of VAC-beta as an anticoagulant and as an inhibitor of phospholipase A2 activity were analyzed and compared to those of VAC-alpha.

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Year:  1989        PMID： 2530088     DOI： 10.1111/j.1432-1033.1989.tb15082.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  23 in total

1.  Proteolytic signals in the primary structure of annexins.

Authors:  Junor A Barnes; Aldrin V Gomes
Journal:  Mol Cell Biochem       Date:  2002-02       Impact factor: 3.396

Review 2.  Annexins: calcium-binding proteins of multi-functional importance?

Authors:  J Römisch; E P Pâques
Journal:  Med Microbiol Immunol       Date:  1991       Impact factor: 3.402

3.  The genetic origin of mouse annexin VIII.

Authors:  M P Fernández; N G Copeland; D J Gilbert; N A Jenkins; R O Morgan
Journal:  Mamm Genome       Date:  1998-01       Impact factor: 2.957

4.  A Vacuole-Associated Annexin Protein, VCaB42, Correlates with the Expansion of Tobacco Cells.

Authors:  D. F. Seals; S. K. Randall
Journal:  Plant Physiol       Date:  1997-10       Impact factor: 8.340

5.  Proteoliposomes with the ability to transport Ca(2+) into the vesicles and hydrolyze phosphosubstrates on their surface.

Authors:  Maytê Bolean; Ana Maria S Simão; Tina Kiffer-Moreira; Marc F Hoylaerts; José Luis Millán; Rosangela Itri; Pietro Ciancaglini
Journal:  Arch Biochem Biophys       Date:  2015-08-29       Impact factor: 4.013

6.  Annexin 5 as a potential regulator of annexin 1 phosphorylation by protein kinase C. In vitro inhibition compared with quantitative data on annexin distribution in human endothelial cells.

Authors:  P Raynal; F Hullin; J M Ragab-Thomas; J Fauvel; H Chap
Journal:  Biochem J       Date:  1993-06-15       Impact factor: 3.857

7.  A 42-kilodalton annexin-like protein is associated with plant vacuoles.

Authors:  D F Seals; M L Parrish; S K Randall
Journal:  Plant Physiol       Date:  1994-12       Impact factor: 8.340

8.  Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.

Authors:  Troels Zakarias Kristiansen; H C Harsha; Mads Grønborg; Anirban Maitra; Akhilesh Pandey
Journal:  J Proteome Res       Date:  2008-10-08       Impact factor: 4.466

9.  Annexins in cardiac tissue: cellular localization and effect on phospholipase activity.

Authors:  M van Bilsen; C P Reutelingsperger; P H Willemsen; R S Reneman; G J van der Vusse
Journal:  Mol Cell Biochem       Date:  1992-10-21       Impact factor: 3.396

10.  Annexin I in female rabbit reproductive organs: varying levels in relation to maturity and pregnancy.

Authors:  F H Tsao; X Chen; X Chen; C H Ts'ao
Journal:  Lipids       Date:  1995-06       Impact factor: 1.880
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