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Literature DB >> 25300665

Flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in mosquito cells.

Abstract

Conditions for flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in infected mosquito cells are described. This approach will streamline investigation of Wolbachia's interactions with host cells and facilitate identification of culture conditions that select for Wolbachia-infected cells.

Entities: CellLine Chemical Disease Species

Keywords: Alphaproteobacteria; Flow cytometry; Intracellular microorganism; Mosquito cell line; Wolbachia

Mesh：

Substances：
Anti-Bacterial Agents

Year: 2014 PMID： 25300665 PMCID： PMC4252651 DOI： 10.1016/j.mimet.2014.09.011

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

23 in total

1. Quantification of bacterial invasion into adherent cells by flow cytometry.

Authors: Stefan Pils; Tim Schmitter; Florian Neske; Christof R Hauck
Journal: J Microbiol Methods Date: 2005-09-26 Impact factor: 2.363

2. Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes.

Authors: Bryan P Tracy; Stefan M Gaida; Eleftherios T Papoutsakis
Journal: Curr Opin Biotechnol Date: 2010-03-05 Impact factor: 9.740

3. In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line.

Authors: S L O'Neill; M M Pettigrew; S P Sinkins; H R Braig; T G Andreadis; R B Tesh
Journal: Insect Mol Biol Date: 1997-02 Impact factor: 3.585

4. Eradication of Culex pipiens fatigans through cytoplasmic incompatibility.

Authors: H Laven
Journal: Nature Date: 1967-10-28 Impact factor: 49.962

5. Flow cytometry of Escherichia coli on microfluidic devices.

Authors: M A McClain; C T Culbertson; S C Jacobson; J M Ramsey
Journal: Anal Chem Date: 2001-11-01 Impact factor: 6.986

6. Mitochondrial DNA variation within and among populations of the mosquito Aedes albopictus.

Authors: S Kambhampati; K S Rai
Journal: Genome Date: 1991-04 Impact factor: 2.166

7. Wolbachia from the planthopper Laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells.

Authors: A M Fallon; G D Baldridge; L A Higgins; B A Witthuhn
Journal: In Vitro Cell Dev Biol Anim Date: 2012-12-28 Impact factor: 2.416

Flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in mosquito cells.

1. Quantification of bacterial invasion into adherent cells by flow cytometry.

2. Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes.

3. In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line.

4. Eradication of Culex pipiens fatigans through cytoplasmic incompatibility.

5. Flow cytometry of Escherichia coli on microfluidic devices.

6. Mitochondrial DNA variation within and among populations of the mosquito Aedes albopictus.

7. Wolbachia from the planthopper Laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells.

8. Determination of DNA content of aquatic bacteria by flow cytometry.

9. Cytological properties of an Aedes albopictus mosquito cell line infected with Wolbachia strain wAlbB.

10. Advances in genetic manipulation of obligate intracellular bacterial pathogens.

1. Strain-specific response to ampicillin in Wolbachia-infected mosquito cell lines.

2. Effects of mimosine on Wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance.

3. Assessment of mitotically inactivated mosquito cell feeder layers produced with mitomycin C.

Review 4. From Mosquito Ovaries to Ecdysone; from Ecdysone to Wolbachia: One Woman's Career in Insect Biology.