Literature DB >> 25300329

TR-FRET Assays for Endogenous Huntingtin Protein Level in Mouse Cells.

Yijian Liang1, Yuwei Yao1, Mingxing Lu1, Jiapeng Hou1, Shenliang Yu1, Boxun Lu2.   

Abstract

High-throughput measurement of huntingtin (Htt) levels is useful for Huntington's disease research. For example, identification of genetic or chemical modifiers that reduce Htt levels by high-throughput screening provides promising strategy for HD drug discovery. In the human cells, high-throughput measurement of Htt levels has been established based on the Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) technology, using the 2B7/MW1 antibody pair. Unfortunately, application of this assay in the mouse cells has been problematic due to discrepancies between TR-FRET signals and Western-blots, possibly caused by non-specific antibody binding. Here we report TR-FRET assays that are able to detect endogenous Htt levels of the mouse striatal cell line (STHdh).

Entities:  

Keywords:  Huntington's disease; TR-FRET; high-throughput technology; protein quantification

Mesh：

Substances：

Year:  2014        PMID： 25300329     DOI： 10.3233/JHD-140104

Source DB:  PubMed          Journal:  J Huntingtons Dis        ISSN： 1879-6397


  6 in total

1.  A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.

Authors:  Yuwei Yao; Xiaotian Cui; Ismael Al-Ramahi; Xiaoli Sun; Bo Li; Jiapeng Hou; Marian Difiglia; James Palacino; Zhi-Ying Wu; Lixiang Ma; Juan Botas; Boxun Lu
Journal:  Elife       Date:  2015-03-04       Impact factor: 8.140

Review 2.  Assays and technologies for developing proteolysis targeting chimera degraders.

Authors:  Xingui Liu; Xuan Zhang; Dongwen Lv; Yaxia Yuan; Guangrong Zheng; Daohong Zhou
Journal:  Future Med Chem       Date:  2020-05-20       Impact factor: 3.808

3.  Quantification of Total and Mutant Huntingtin Protein Levels in Biospecimens Using a Novel alphaLISA Assay.

Authors:  Barbara Baldo; Muhammad Umar Sajjad; Rachel Y Cheong; Julie Bigarreau; Ravi Vijayvargia; Catriona McLean; Anselme L Perrier; Ihn Sik Seong; Glenda Halliday; Åsa Petersén; Deniz Kirik
Journal:  eNeuro       Date:  2018-10-10

4.  A high-throughput-compatible assay to measure the degradation of endogenous Huntingtin proteins.

Authors:  Peng Wu; Ming-Xing Lu; Xiao-Tian Cui; He-Qing Yang; Shen-Liang Yu; Jian-Bin Zhu; Xiao-Li Sun; Boxun Lu
Journal:  Acta Pharmacol Sin       Date:  2016-06-06       Impact factor: 6.150

5.  Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models.

Authors:  Meng Yu; Yuhua Fu; Yijiang Liang; Haikun Song; Yao Yao; Peng Wu; Yuwei Yao; Yuyin Pan; Xue Wen; Lixiang Ma; Saiyin Hexige; Yu Ding; Shouqing Luo; Boxun Lu
Journal:  Cell Res       Date:  2017-10-13       Impact factor: 25.617

6.  Targeting Gpr52 lowers mutant HTT levels and rescues Huntington's disease-associated phenotypes.

Authors:  Haikun Song; Hexuan Li; Shimeng Guo; Yuyin Pan; Yuhua Fu; Zijian Zhou; Zhaoyang Li; Xue Wen; Xiaoli Sun; Bingqing He; Haifeng Gu; Quan Zhao; Cen Wang; Ping An; Shouqing Luo; Youhong Hu; Xin Xie; Boxun Lu
Journal:  Brain       Date:  2018-06-01       Impact factor: 13.501
  6 in total

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