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Literature DB >> 25299574

Sortase-mediated ligations for the site-specific modification of proteins.

Abstract

Sortase-mediated ligation (SML) is one of the most commonly used techniques for the site-specific modification of proteins. Here, a brief overview on advantages and limitations of this technology in comparison with other chemoselective protein modification techniques is provided and successful approaches that extend the application range of SML are discussed.

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Year: 2014 PMID： 25299574 DOI： 10.1016/j.cbpa.2014.09.020

Source DB: PubMed Journal: Curr Opin Chem Biol ISSN： 1367-5931 Impact factor: 8.822

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Cited

23 in total

1. Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag.

Authors: Sierra A Reed; David A Brzovic; Savanna S Takasaki; Kristina V Boyko; John M Antos
Journal: Bioconjug Chem Date: 2020-04-23 Impact factor: 4.774

2. Hepta-Mutant Staphylococcus aureus Sortase A (SrtA_7m) as a Tool for in Vivo Protein Labeling in Caenorhabditis elegans.

Authors: Qin Wu; Hidde L Ploegh; Matthias C Truttmann
Journal: ACS Chem Biol Date: 2017-01-18 Impact factor: 5.100

Review 3. A molecular engineering toolbox for the structural biologist.

Authors: Galia T Debelouchina; Tom W Muir
Journal: Q Rev Biophys Date: 2017-01 Impact factor: 5.318

4. Optimization of sortase A ligation for flexible engineering of complex protein systems.

Authors: Jess Li; Yue Zhang; Olivier Soubias; Domarin Khago; Fa-An Chao; Yifei Li; Katherine Shaw; R Andrew Byrd
Journal: J Biol Chem Date: 2020-01-23 Impact factor: 5.157

Review 5. Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.

Authors: Alex W Jacobitz; Michele D Kattke; Jeff Wereszczynski; Robert T Clubb
Journal: Adv Protein Chem Struct Biol Date: 2017-06-05 Impact factor: 3.507

6. Kinetics and Optimization of the Lysine-Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae.

Authors: Christopher K Sue; Scott A McConnell; Ken Ellis-Guardiola; John M Muroski; Rachel A McAllister; Justin Yu; Ana I Alvarez; Chungyu Chang; Rachel R Ogorzalek Loo; Joseph A Loo; Hung Ton-That; Robert T Clubb
Journal: Bioconjug Chem Date: 2020-05-27 Impact factor: 4.774

Sortase-mediated ligations for the site-specific modification of proteins.

1. Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag.

2. Hepta-Mutant Staphylococcus aureus Sortase A (SrtA_7m) as a Tool for in Vivo Protein Labeling in Caenorhabditis elegans.

Review 3. A molecular engineering toolbox for the structural biologist.

4. Optimization of sortase A ligation for flexible engineering of complex protein systems.

Review 5. Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.

6. Kinetics and Optimization of the Lysine-Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae.

7. Site-specific protein labeling via sortase-mediated transpeptidation.

Review 8. Recent advances in sortase-catalyzed ligation methodology.

Review 9. Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.

Review 10. Enzymatic Methods for the Site-Specific Radiolabeling of Targeting Proteins.

Sortase-mediated ligations for the site-specific modification of proteins.

1. Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag.

2. Hepta-Mutant Staphylococcus aureus Sortase A (SrtA7m) as a Tool for in Vivo Protein Labeling in Caenorhabditis elegans.

Review 3. A molecular engineering toolbox for the structural biologist.

4. Optimization of sortase A ligation for flexible engineering of complex protein systems.

Review 5. Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.

6. Kinetics and Optimization of the Lysine-Isopeptide Bond Forming Sortase Enzyme from Corynebacterium diphtheriae.

7. Site-specific protein labeling via sortase-mediated transpeptidation.

Review 8. Recent advances in sortase-catalyzed ligation methodology.

Review 9. Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.

Review 10. Enzymatic Methods for the Site-Specific Radiolabeling of Targeting Proteins.

2. Hepta-Mutant Staphylococcus aureus Sortase A (SrtA_7m) as a Tool for in Vivo Protein Labeling in Caenorhabditis elegans.