BACKGROUND: The withdrawal of malathion in the European Union in 2009 resulted in a large increase in lambda-cyhalothrin applications for the control of the Mediterranean fruit fly, Ceratitis capitata, in Spanish citrus crops. RESULTS: Spanish field populations of C. capitata have developed resistance to lambda-cyhalothrin (6-14-fold), achieving LC50 values (129-287 ppm) higher than the recommended concentration for field treatments (125 ppm). These results contrast with the high susceptibility to lambda-cyhalothrin found in three Tunisian field populations. We have studied the mechanism of resistance in the laboratory-selected resistant strain W-1Kλ (205-fold resistance). Bioassays with synergists showed that resistance was almost completely suppressed by the P450 inhibitor PBO. The study of the expression of 53 P450 genes belonging to the CYP4, CYP6, CYP9 and CYP12 families in C. capitata revealed that CYP6A51 was overexpressed (13-18-fold) in the resistant strain. The W-1Kλ strain also showed high levels of cross-resistance to etofenprox (240-fold) and deltamethrin (150-fold). CONCLUSION: Field-evolved resistance to lambda-cyhalothrin has been found in C. capitata. Metabolic resistance mediated by P450 appears to be the main resistance mechanism in the resistant strain W-1Kλ. The levels of cross-resistance found may compromise the effectiveness of other pyrethroids for the control of this species.
BACKGROUND: The withdrawal of malathion in the European Union in 2009 resulted in a large increase in lambda-cyhalothrin applications for the control of the Mediterranean fruit fly, Ceratitis capitata, in Spanish citrus crops. RESULTS: Spanish field populations of C. capitata have developed resistance to lambda-cyhalothrin (6-14-fold), achieving LC50 values (129-287 ppm) higher than the recommended concentration for field treatments (125 ppm). These results contrast with the high susceptibility to lambda-cyhalothrin found in three Tunisian field populations. We have studied the mechanism of resistance in the laboratory-selected resistant strain W-1Kλ (205-fold resistance). Bioassays with synergists showed that resistance was almost completely suppressed by the P450 inhibitor PBO. The study of the expression of 53 P450 genes belonging to the CYP4, CYP6, CYP9 and CYP12 families in C. capitata revealed that CYP6A51 was overexpressed (13-18-fold) in the resistant strain. The W-1Kλ strain also showed high levels of cross-resistance to etofenprox (240-fold) and deltamethrin (150-fold). CONCLUSION: Field-evolved resistance to lambda-cyhalothrin has been found in C. capitata. Metabolic resistance mediated by P450 appears to be the main resistance mechanism in the resistant strain W-1Kλ. The levels of cross-resistance found may compromise the effectiveness of other pyrethroids for the control of this species.
Authors: P Stupp; M Rakes; D C Oliveira; L N Martins; F C S Geisler; L P Ribeiro; D E Nava; D Bernardi Journal: Neotrop Entomol Date: 2020-01-06 Impact factor: 1.434
Authors: Ana Guillem-Amat; Elena López-Errasquín; Javier Castells-Sierra; Lucas Sánchez; Félix Ortego Journal: Pest Manag Sci Date: 2021-12-21 Impact factor: 4.462
Authors: Alexie Papanicolaou; Marc F Schetelig; Peter Arensburger; Peter W Atkinson; Joshua B Benoit; Kostas Bourtzis; Pedro Castañera; John P Cavanaugh; Hsu Chao; Christopher Childers; Ingrid Curril; Huyen Dinh; HarshaVardhan Doddapaneni; Amanda Dolan; Shannon Dugan; Markus Friedrich; Giuliano Gasperi; Scott Geib; Georgios Georgakilas; Richard A Gibbs; Sarah D Giers; Ludvik M Gomulski; Miguel González-Guzmán; Ana Guillem-Amat; Yi Han; Artemis G Hatzigeorgiou; Pedro Hernández-Crespo; Daniel S T Hughes; Jeffery W Jones; Dimitra Karagkouni; Panagiota Koskinioti; Sandra L Lee; Anna R Malacrida; Mosè Manni; Kostas Mathiopoulos; Angela Meccariello; Shwetha C Murali; Terence D Murphy; Donna M Muzny; Georg Oberhofer; Félix Ortego; Maria D Paraskevopoulou; Monica Poelchau; Jiaxin Qu; Martin Reczko; Hugh M Robertson; Andrew J Rosendale; Andrew E Rosselot; Giuseppe Saccone; Marco Salvemini; Grazia Savini; Patrick Schreiner; Francesca Scolari; Paolo Siciliano; Sheina B Sim; George Tsiamis; Enric Ureña; Ioannis S Vlachos; John H Werren; Ernst A Wimmer; Kim C Worley; Antigone Zacharopoulou; Stephen Richards; Alfred M Handler Journal: Genome Biol Date: 2016-09-22 Impact factor: 13.583
Authors: Luis Hernández-Pelegrín; Ángel Llopis-Giménez; Cristina Maria Crava; Félix Ortego; Pedro Hernández-Crespo; Vera I D Ros; Salvador Herrero Journal: Viruses Date: 2022-03-17 Impact factor: 5.048