Silvia Carraro1, Giuseppe Giordano1, Paola Pirillo1, Michela Maretti1, Fabiano Reniero2, Paola E Cogo3, Giorgio Perilongo1, Matteo Stocchero4, Eugenio Baraldi5. 1. Women's and Children's Health Department, University of Padova, Padova, Italy. 2. European Commission, Joint Research Center, Institute for Health and Consumer Protection (IHCP), System Toxicology Unit (ST), Ispra (VA), Italy. 3. Anesthesia and Cardiac Intensive Care, Bambino Gesù Pediatric Hospital, Rome, Italy. 4. S-IN Soluzioni Informatiche, Vicenza, Italy. 5. Women's and Children's Health Department, University of Padova, Padova, Italy. Electronic address: baraldi@pediatria.unipd.it.
Abstract
OBJECTIVES: To assess a group of adolescents with bronchopulmonary dysplasia (BPD) from a biochemical-metabolic standpoint, applying the metabolomic approach to studying their exhaled breath condensate (EBC). STUDY DESIGN: Twenty adolescents with BPD (mean age 14.8 years) and 15 healthy controls (mean age 15.2 years) were recruited for EBC collection, exhaled nitric oxide measurement, and spirometry. The EBC samples were analyzed using a metabolomic approach based on mass spectrometry. The obtained spectra were analyzed using multivariate statistical analysis tools. RESULTS: A reliable Orthogonal Projections to Latent Structures-Discriminant Analysis model showed a clear discrimination between cases of BPD and healthy controls (R(2) = 0.95 and Q(2) = 0.92). The search for putative biomarkers identified an altered complex lipid profile in the adolescents with BPD. CONCLUSIONS: The metabolomic analysis of EBC distinguishes cases of BPD from healthy individuals, suggesting that the lung of survivors of BPD is characterized by long-term metabolic abnormalities. The search for putative biomarkers indicated a possible role of an altered surfactant composition, which may persist far beyond infancy.
OBJECTIVES: To assess a group of adolescents with bronchopulmonary dysplasia (BPD) from a biochemical-metabolic standpoint, applying the metabolomic approach to studying their exhaled breath condensate (EBC). STUDY DESIGN: Twenty adolescents with BPD (mean age 14.8 years) and 15 healthy controls (mean age 15.2 years) were recruited for EBC collection, exhaled nitric oxide measurement, and spirometry. The EBC samples were analyzed using a metabolomic approach based on mass spectrometry. The obtained spectra were analyzed using multivariate statistical analysis tools. RESULTS: A reliable Orthogonal Projections to Latent Structures-Discriminant Analysis model showed a clear discrimination between cases of BPD and healthy controls (R(2) = 0.95 and Q(2) = 0.92). The search for putative biomarkers identified an altered complex lipid profile in the adolescents with BPD. CONCLUSIONS: The metabolomic analysis of EBC distinguishes cases of BPD from healthy individuals, suggesting that the lung of survivors of BPD is characterized by long-term metabolic abnormalities. The search for putative biomarkers indicated a possible role of an altered surfactant composition, which may persist far beyond infancy.
Authors: Michael R La Frano; Johannes F Fahrmann; Dmitry Grapov; Theresa L Pedersen; John W Newman; Oliver Fiehn; Mark A Underwood; Karen Mestan; Robin H Steinhorn; Stephen Wedgwood Journal: Am J Physiol Lung Cell Mol Physiol Date: 2018-08-16 Impact factor: 5.464
Authors: Katarzyna Kosik; Anna Sowińska; Agnieszka Seremak-Mrozikiewicz; Jasmine A Abu-Amara; Salwan R Al-Saad; Lukasz M Karbowski; Katarzyna Gryczka; Grażyna Kurzawińska; Marta Szymankiewicz-Bręborowicz; Krzysztof Drews; Dawid Szpecht Journal: Mol Cell Biochem Date: 2022-03-01 Impact factor: 3.396