Literature DB >> 25291978

NMR study of non-structural proteins--part II: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Venezuelan equine encephalitis virus (VEEV).

Garyfallia I Makrynitsa1, Dioni Ntonti1, Konstantinos D Marousis1, Aikaterini C Tsika1, Julie Lichière2,3, Nicolas Papageorgiou2,3, Bruno Coutard2,3, Detlef Bentrop4, Georgios A Spyroulias5.   

Abstract

Macro domains consist of 130-190 amino acid residues and appear to be highly conserved in all kingdoms of life. Intense research on this field has shown that macro domains bind ADP-ribose and other similar molecules, but their exact function still remains intangible. Macro domains are highly conserved in the Alphavirus genus and the Venezuelan equine encephalitis virus (VEEV) is a member of this genus that causes fatal encephalitis to equines and humans. In this study we report the high yield recombinant expression and preliminary solution NMR study of the macro domain of VEEV. An almost complete sequence-specific assignment of its (1)H, (15)N and (13)C resonances was obtained and its secondary structure predicted by TALOS+. The protein shows a unique mixed α/β-fold.

Entities:  

Keywords:  ADP-ribose-binding module; Alphavirus; NMR spectroscopy; Recombinant protein expression; Venezuelan equine encephalitis virus; Viral macro domains

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Substances：

Year:  2014        PMID： 25291978     DOI： 10.1007/s12104-014-9584-9

Source DB:  PubMed          Journal:  Biomol NMR Assign        ISSN： 1874-270X            Impact factor:   0.746


  5 in total

1.  Binding Adaptation of GS-441524 Diversifies Macro Domains and Downregulates SARS-CoV-2 de-MARylation Capacity.

Authors:  Aikaterini C Tsika; Angelo Gallo; Nikolaos K Fourkiotis; Aikaterini I Argyriou; Sridhar Sreeramulu; Frank Löhr; Vladimir V Rogov; Christian Richter; Verena Linhard; Santosh L Gande; Nadide Altincekic; Robin Krishnathas; Isam Elamri; Harald Schwalbe; Jan Wollenhaupt; Manfred S Weiss; Georgios A Spyroulias
Journal:  J Mol Biol       Date:  2022-07-15       Impact factor: 6.151

Review 2.  The Enigmatic Alphavirus Non-Structural Protein 3 (nsP3) Revealing Its Secrets at Last.

Authors:  Benjamin Götte; Lifeng Liu; Gerald M McInerney
Journal:  Viruses       Date:  2018-02-28       Impact factor: 5.048

3.  Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose.

Authors:  Garyfallia I Makrynitsa; Dioni Ntonti; Konstantinos D Marousis; Maria Birkou; Minos-Timotheos Matsoukas; Sam Asami; Detlef Bentrop; Nicolas Papageorgiou; Bruno Canard; Bruno Coutard; Georgios A Spyroulias
Journal:  J Struct Biol       Date:  2019-02-27       Impact factor: 2.867

4.  NMR study of macro domains (MDs) from betacoronavirus: backbone resonance assignments of SARS-CoV and MERS-CoV MDs in the free and the ADPr-bound state.

Authors:  Aikaterini C Tsika; Nikolaos K Fourkiotis; Periklis Charalampous; Angelo Gallo; Georgios A Spyroulias
Journal:  Biomol NMR Assign       Date:  2021-10-22       Impact factor: 0.731

Review 5.  The Putative Roles and Functions of Indel, Repetition and Duplication Events in Alphavirus Non-Structural Protein 3 Hypervariable Domain (nsP3 HVD) in Evolution, Viability and Re-Emergence.

Authors:  Nurshariza Abdullah; Nafees Ahemad; Konstantinos Aliazis; Jasmine Elanie Khairat; Thong Chuan Lee; Siti Aisyah Abdul Ahmad; Nur Amelia Azreen Adnan; Nur Omar Macha; Sharifah Syed Hassan
Journal:  Viruses       Date:  2021-05-28       Impact factor: 5.048
  5 in total

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