Literature DB >> 25287845

Step by step manipulation of the CryoCapsule with HPM high pressure freezers.

Xavier Heiligenstein1, Ilse Hurbain2, Cédric Delevoye1, Jean Salamero3, Claude Antony4, Graca Raposo2.   

Abstract

The CryoCapsule is a tool dedicated to correlative light to electron microscopy experiments. Focused on simplifying the specimen manipulation throughout the entire workflow from live-cell imaging to freeze substitution following cryofixation by high pressure freezing, we introduce here a step by step procedure to use the CryoCapsule either with the high pressure freezing machines: HPM010 or the HPM100.
© 2014 Elsevier Inc. All rights reserved.

Keywords:  Correlative light and electron microscopy; CryoCapsule; HPM010; HPM100; High pressure freezing; Live-cell imaging

Mesh：

Substances：

Year:  2014        PMID： 25287845     DOI： 10.1016/B978-0-12-801075-4.00012-4

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN： 0091-679X            Impact factor:   1.441


  2 in total

1.  BLOC-1 Brings Together the Actin and Microtubule Cytoskeletons to Generate Recycling Endosomes.

Authors:  Cédric Delevoye; Xavier Heiligenstein; Léa Ripoll; Floriane Gilles-Marsens; Megan K Dennis; Ricardo A Linares; Laura Derman; Avanti Gokhale; Etienne Morel; Victor Faundez; Michael S Marks; Graça Raposo
Journal:  Curr Biol       Date:  2015-12-24       Impact factor: 10.834

2.  Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome.

Authors:  Alexandra Botté; Jeanne Lainé; Laura Xicota; Xavier Heiligenstein; Gaëlle Fontaine; Amal Kasri; Isabelle Rivals; Pollyanna Goh; Orestis Faklaris; Jack-Christophe Cossec; Etienne Morel; Anne-Sophie Rebillat; Dean Nizetic; Graça Raposo; Marie-Claude Potier
Journal:  Acta Neuropathol Commun       Date:  2020-06-24       Impact factor: 7.801
  2 in total

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