Literature DB >> 25287337

Nuclear transfer and transgenesis in the pig.

Mayuko Kurome1, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, Eckhard Wolf.   

Abstract

Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, SCNT using in vitro matured oocytes, and laparoscopic embryo transfer.

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Year:  2015        PMID： 25287337     DOI： 10.1007/978-1-4939-1594-1_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters.

Authors:  Elisabeth Kemter; Christian M Cohrs; Matthias Schäfer; Marion Schuster; Klaus Steinmeyer; Lelia Wolf-van Buerck; Andrea Wolf; Annegret Wuensch; Mayuko Kurome; Barbara Kessler; Valeri Zakhartchenko; Matthias Loehn; Yuri Ivashchenko; Jochen Seissler; Anke M Schulte; Stephan Speier; Eckhard Wolf
Journal:  Diabetologia       Date:  2017-03-18       Impact factor: 10.122

2.  Viable pigs with a conditionally-activated oncogenic KRAS mutation.

Authors:  Shun Li; Marlene Edlinger; Anja Saalfrank; Krzysztof Flisikowski; Alexander Tschukes; Mayuko Kurome; Valeri Zakhartchenko; Barbara Kessler; Dieter Saur; Alexander Kind; Eckhard Wolf; Angelika Schnieke; Tatiana Flisikowska
Journal:  Transgenic Res       Date:  2015-02-26       Impact factor: 2.788

3.  A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies.

Authors:  Tatiana Flisikowska; Jerome Egli; Angelika Schnieke; Antonio Iglesias; Krzysztof Flisikowski; Marlene Stumbaum; Erich Küng; Martin Ebeling; Roland Schmucki; Guy Georges; Thomas Singer; Mayuko Kurome; Barbara Kessler; Valeri Zakhartchenko; Eckhard Wolf; Felix Weber
Journal:  Nat Biomed Eng       Date:  2022-09-22       Impact factor: 29.234

4.  Direct introduction of gene constructs into the pronucleus-like structure of cloned embryos: a new strategy for the generation of genetically modified pigs.

Authors:  Mayuko Kurome; Simon Leuchs; Barbara Kessler; Elisabeth Kemter; Eva-Maria Jemiller; Beatrix Foerster; Nikolai Klymiuk; Valeri Zakhartchenko; Eckhard Wolf
Journal:  Transgenic Res       Date:  2016-12-10       Impact factor: 2.788

5.  Somatic gene editing ameliorates skeletal and cardiac muscle failure in pig and human models of Duchenne muscular dystrophy.

Authors:  A Moretti; L Fonteyne; F Giesert; P Hoppmann; A B Meier; T Bozoglu; A Baehr; C M Schneider; D Sinnecker; K Klett; T Fröhlich; F Abdel Rahman; T Haufe; S Sun; V Jurisch; B Kessler; R Hinkel; R Dirschinger; E Martens; C Jilek; A Graf; S Krebs; G Santamaria; M Kurome; V Zakhartchenko; B Campbell; K Voelse; A Wolf; T Ziegler; S Reichert; S Lee; F Flenkenthaler; T Dorn; I Jeremias; H Blum; A Dendorfer; A Schnieke; S Krause; M C Walter; N Klymiuk; K L Laugwitz; E Wolf; W Wurst; C Kupatt
Journal:  Nat Med       Date:  2020-01-27       Impact factor: 53.440

Review 6.  A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.

Authors:  Claudia Springer; Eckhard Wolf; Kilian Simmet
Journal:  J Dev Biol       Date:  2021-04-02

7.  Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation.

Authors:  Masahito Watanabe; Kazuhiro Umeyama; Kazuaki Nakano; Hitomi Matsunari; Toru Fukuda; Kei Matsumoto; Susumu Tajiri; Shuichiro Yamanaka; Koki Hasegawa; Kazutoshi Okamoto; Ayuko Uchikura; Shuko Takayanagi; Masaki Nagaya; Takashi Yokoo; Hiromitsu Nakauchi; Hiroshi Nagashima
Journal:  Lab Invest       Date:  2022-01-03       Impact factor: 5.502

8.  Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs.

Authors:  Andrea Bähr; Tobias Käser; Elisabeth Kemter; Wilhelm Gerner; Mayuko Kurome; Wiebke Baars; Nadja Herbach; Kirsti Witter; Annegret Wünsch; Stephanie C Talker; Barbara Kessler; Hiroshi Nagashima; Armin Saalmüller; Reinhard Schwinzer; Eckhard Wolf; Nikolai Klymiuk
Journal:  PLoS One       Date:  2016-05-13       Impact factor: 3.240

9.  Fetal Sex Modulates Developmental Response to Maternal Malnutrition.

Authors:  Antonio Gonzalez-Bulnes; Laura Torres-Rovira; Susana Astiz; Cristina Ovilo; Raul Sanchez-Sanchez; Ernesto Gomez-Fidalgo; Mariluz Perez-Solana; Mercedes Martin-Lluch; Consuelo Garcia-Contreras; Marta Vazquez-Gomez
Journal:  PLoS One       Date:  2015-11-06       Impact factor: 3.240

10.  Generation of α1,3-galactosyltransferase and cytidine monophospho-N-acetylneuraminic acid hydroxylase gene double-knockout pigs.

Authors:  Shuji Miyagawa; Hitomi Matsunari; Masahito Watanabe; Kazuaki Nakano; Kazuhiro Umeyama; Rieko Sakai; Shuko Takayanagi; Toki Takeishi; Tooru Fukuda; Sayaka Yashima; Akira Maeda; Hiroshi Eguchi; Hiroomi Okuyama; Masaki Nagaya; Hiroshi Nagashima
Journal:  J Reprod Dev       Date:  2015-07-26       Impact factor: 2.214
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