Literature DB >> 25286383

Canonical histone H2Ba and H2A.X dimerize in an opposite genomic localization to H2A.Z/H2B.Z dimers in Toxoplasma gondii.

Silvina S Bogado1, María C Dalmasso1, Agustina Ganuza2, Kami Kim3, William J Sullivan4, Sergio O Angel1, Laura Vanagas5.   

Abstract

Histone H2Ba of Toxoplasma gondii was expressed as recombinant protein (rH2Ba) and used to generate antibody in mouse that is highly specific. Antibody recognizing rH2Ba detects a single band in tachyzoite lysate of the expected molecular weight (12kDa). By indirect immunofluorescence (IFA) in in vitro grown tachyzoites and bradyzoites, the signal was detected only in the parasite nucleus. The nucleosome composition of H2Ba was determined through co-immunoprecipitation assays. H2Ba was detected in the same immunocomplex as H2A.X, but not with H2A.Z. Through chromatin immunoprecipitation (ChIP) assays and qPCR, it was observed that H2Ba is preferentially located at promoters of inactive genes and silent regions, accompanying H2A.X and opposed to H2A.Z/H2B.Z dimers.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Canonical histones; ChIP; Chromatin; Epigenetics; Nucleosome; Toxoplasma

Mesh：

Substances：

Year:  2014        PMID： 25286383      PMCID： PMC4254167          DOI： 10.1016/j.molbiopara.2014.09.009

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  41 in total

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Review 7.  Congenital toxoplasmosis.

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Journal:  PLoS Pathog       Date:  2011-02-17       Impact factor: 6.823

9.  A unified phylogeny-based nomenclature for histone variants.

Authors:  Paul B Talbert; Kami Ahmad; Geneviève Almouzni; Juan Ausió; Frederic Berger; Prem L Bhalla; William M Bonner; W Zacheus Cande; Brian P Chadwick; Simon W L Chan; George A M Cross; Liwang Cui; Stefan I Dimitrov; Detlef Doenecke; José M Eirin-López; Martin A Gorovsky; Sandra B Hake; Barbara A Hamkalo; Sarah Holec; Steven E Jacobsen; Kinga Kamieniarz; Saadi Khochbin; Andreas G Ladurner; David Landsman; John A Latham; Benjamin Loppin; Harmit S Malik; William F Marzluff; John R Pehrson; Jan Postberg; Robert Schneider; Mohan B Singh; M Mitchell Smith; Eric Thompson; Maria-Elena Torres-Padilla; David John Tremethick; Bryan M Turner; Jakob Harm Waterborg; Heike Wollmann; Ramesh Yelagandula; Bing Zhu; Steven Henikoff
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Review 10.  Identification and interrogation of combinatorial histone modifications.

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  3 in total

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Review 2.  Emerging Therapeutic Targets Against Toxoplasma gondii: Update on DNA Repair Response Inhibitors and Genotoxic Drugs.

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  3 in total

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