Literature DB >> 25285338

Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase.

Alhosna Benjdia1, Korbinian Heil, Andreas Winkler, Thomas Carell, Ilme Schlichting.   

Abstract

The radical SAM enzyme, spore photoproduct lyase, requires an H-atom transfer (HAT) pathway to catalyze DNA repair. By rational engineering, we demonstrate that it is possible to rewire its HAT pathway, a first step toward the development of novel catalysts based on the radical SAM enzyme scaffold.

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Year:  2014        PMID： 25285338     DOI： 10.1039/c4cc05158k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  9 in total

1.  Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme-The spore photoproduct lyase.

Authors:  Linlin Yang; Yajun Jian; Peter Setlow; Lei Li
Journal:  DNA Repair (Amst)       Date:  2017-03-06

2.  Kinetic Isotope Effects and Hydrogen/Deuterium Exchange Reveal Large Conformational Changes During the Catalysis of the Clostridium acetobutylicum Spore Photoproduct Lyase.

Authors:  Linlin Yang; Jagat Adhikari; Michael L Gross; Lei Li
Journal:  Photochem Photobiol       Date:  2017-01-30       Impact factor: 3.421

3.  Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis.

Authors:  Alhosna Benjdia; Alain Guillot; Pauline Ruffié; Jérôme Leprince; Olivier Berteau
Journal:  Nat Chem       Date:  2017-02-06       Impact factor: 24.427

4.  Radical SAM Enzyme Spore Photoproduct Lyase: Properties of the Ω Organometallic Intermediate and Identification of Stable Protein Radicals Formed during Substrate-Free Turnover.

Authors:  Adrien Pagnier; Hao Yang; Richard J Jodts; Christopher D James; Eric M Shepard; Stella Impano; William E Broderick; Brian M Hoffman; Joan B Broderick
Journal:  J Am Chem Soc       Date:  2020-10-15       Impact factor: 15.419

Review 5.  Photochemistry and Photobiology of the Spore Photoproduct: A 50-Year Journey.

Authors:  Peter Setlow; Lei Li
Journal:  Photochem Photobiol       Date:  2015-09-20       Impact factor: 3.421

6.  Insights into the Activity Change of Spore Photoproduct Lyase Induced by Mutations at a Peripheral Glycine Residue.

Authors:  Linlin Yang; Lei Li
Journal:  Front Chem       Date:  2017-03-28       Impact factor: 5.221

7.  Mechanistic Investigations of PoyD, a Radical S-Adenosyl-l-methionine Enzyme Catalyzing Iterative and Directional Epimerizations in Polytheonamide A Biosynthesis.

Authors:  Aubérie Parent; Alhosna Benjdia; Alain Guillot; Xavier Kubiak; Clémence Balty; Benjamin Lefranc; Jérôme Leprince; Olivier Berteau
Journal:  J Am Chem Soc       Date:  2018-02-06       Impact factor: 15.419

Review 8.  Radical SAM Enzymes in the Biosynthesis of Ribosomally Synthesized and Post-translationally Modified Peptides (RiPPs).

Authors:  Alhosna Benjdia; Clémence Balty; Olivier Berteau
Journal:  Front Chem       Date:  2017-11-08       Impact factor: 5.221

9.  Analysis of Electrochemical Properties of S-Adenosyl-l-methionine and Implications for Its Role in Radical SAM Enzymes.

Authors:  Sven A Miller; Vahe Bandarian
Journal:  J Am Chem Soc       Date:  2019-07-08       Impact factor: 15.419
  9 in total

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