I Garzón1, C A Alfonso-Rodríguez2, C Martínez-Gómez3, V Carriel1, M A Martin-Piedra1, R Fernández-Valadés4, M C Sánchez-Quevedo1, M Alaminos5. 1. Department of Histology (Tissue Engineering Group), University of Granada, Spain; Instituto de Investigación Biosanitaria ibs. Granada, Spain. 2. Department of Histology (Tissue Engineering Group), University of Granada, Spain; Instituto de Investigación Biosanitaria ibs. Granada, Spain; PhD programme in Biomedicine, University of Granada, Spain. 3. PhD programme in Clinical Medicine and Public Health, University of Granada, Spain. 4. Instituto de Investigación Biosanitaria ibs. Granada, Spain; Division of Pediatric Surgery, University Hospital Virgen de las Nieves, Granada, Spain. 5. Department of Histology (Tissue Engineering Group), University of Granada, Spain; Instituto de Investigación Biosanitaria ibs. Granada, Spain. Electronic address: malaminos@ugr.es.
Abstract
INTRODUCTION: Human umbilical cord stem cells have inherent differentiation capabilities and potential usefulness in regenerative medicine. However, the epithelial differentiation capability and the heterogeneity of these cells have not been fully explored to the date. METHODS: We analyzed the expression of several undifferentiation and epithelial markers in cells located in situ in different zones of the umbilical cord -in situ analysis- and in primary ex vivo cell cultures of Wharton's jelly stem cells by microarray and immunofluorescence. RESULTS: Our results demonstrated that umbilical cord cells were heterogeneous and had intrinsic capability to express in situ stem cell markers, CD90 and CD105 and the epithelial markers cytokeratins 3, 4, 7, 8, 12, 13, 19, desmoplakin and zonula occludens 1 as determined by microarray and immunofluorescence, and most of these markers remained expressed after transferring the cells from the in situ to the ex vivo cell culture conditions. However, important differences were detected among some cell types in the umbilical cord, with subvascular zone cells showing less expression of stem cell markers and cells in Wharton's jelly and the amnioblastic zones showing the highest expression of stem cells and epithelial markers. CONCLUSIONS: These results suggest that umbilical cord mesenchymal cells have intrinsic potential to express relevant epithelial markers, and support the idea that they could be used as alternative cell sources for epithelial tissue engineering.
INTRODUCTION:Human umbilical cord stem cells have inherent differentiation capabilities and potential usefulness in regenerative medicine. However, the epithelial differentiation capability and the heterogeneity of these cells have not been fully explored to the date. METHODS: We analyzed the expression of several undifferentiation and epithelial markers in cells located in situ in different zones of the umbilical cord -in situ analysis- and in primary ex vivo cell cultures of Wharton's jelly stem cells by microarray and immunofluorescence. RESULTS: Our results demonstrated that umbilical cord cells were heterogeneous and had intrinsic capability to express in situ stem cell markers, CD90 and CD105 and the epithelial markers cytokeratins 3, 4, 7, 8, 12, 13, 19, desmoplakin and zonula occludens 1 as determined by microarray and immunofluorescence, and most of these markers remained expressed after transferring the cells from the in situ to the ex vivo cell culture conditions. However, important differences were detected among some cell types in the umbilical cord, with subvascular zone cells showing less expression of stem cell markers and cells in Wharton's jelly and the amnioblastic zones showing the highest expression of stem cells and epithelial markers. CONCLUSIONS: These results suggest that umbilical cord mesenchymal cells have intrinsic potential to express relevant epithelial markers, and support the idea that they could be used as alternative cell sources for epithelial tissue engineering.
Authors: D Durand-Herrera; F Campos; B D Jaimes-Parra; J D Sánchez-López; R Fernández-Valadés; M Alaminos; A Campos; V Carriel Journal: Histochem Cell Biol Date: 2018-06-11 Impact factor: 4.304
Authors: Laura García-Martínez; Fernando Campos; Carlos Godoy-Guzmán; María Del Carmen Sánchez-Quevedo; Ingrid Garzón; Miguel Alaminos; Antonio Campos; Víctor Carriel Journal: Histochem Cell Biol Date: 2016-09-01 Impact factor: 4.304
Authors: Ingrid Garzón; Jesus Chato-Astrain; Carmen González-Gallardo; Ana Ionescu; Juan de la Cruz Cardona; Miguel Mateu; Carmen Carda; María Del Mar Pérez; Miguel Ángel Martín-Piedra; Miguel Alaminos Journal: Front Bioeng Biotechnol Date: 2020-06-19
Authors: V Shablii; M Kuchma; H Svitina; I Skrypkina; P Areshkov; V Kyryk; T Bukreieva; V Nikulina; Iu Shablii; G Lobyntseva Journal: Biomed Res Int Date: 2019-07-15 Impact factor: 3.411
Authors: Cristina Blanco-Elices; Jesús Chato-Astrain; Alberto González-González; David Sánchez-Porras; Víctor Carriel; Ricardo Fernández-Valadés; María Del Carmen Sánchez-Quevedo; Miguel Alaminos; Ingrid Garzón Journal: J Pers Med Date: 2022-04-18
Authors: Ingrid Garzón; Boris Damián Jaimes-Parra; Manrique Pascual-Geler; José Manuel Cózar; María Del Carmen Sánchez-Quevedo; María Auxiliadora Mosquera-Pacheco; Indalecio Sánchez-Montesinos; Ricardo Fernández-Valadés; Fernando Campos; Miguel Alaminos Journal: Polymers (Basel) Date: 2021-05-13 Impact factor: 4.329