Gökhan Ozdemir1, Metin Kılınç2, Yusuf Ergün3, Elif Sahin2. 1. Department of Ophthalmology, Faculty of Medicine, Kahramanmaraş Sütçüimam University, Kahramanmaraş, Turkiye. Electronic address: gozdemir@hotmail.com. 2. Department of Biochemistry, Faculty of Medicine, Kahramanmaraş Sütçüimam University, Kahramanmaraş, Turkiye. 3. Department of Pharmacology, Faculty of Medicine, Kahramanmaraş Sütçüimam University, Kahramanmaraş, Turkiye.
Abstract
OBJECTIVE: To evaluate the role of rapamycin in the prevention of diabetic oxidative stress and the regulation of angiogenic factors. DESIGN: Experimental animal study. METHODS: Diabetes was induced in 20 adult male Wistar rats by a single intraperitoneal administration of streptozotocin (60 mg/kg). Rats were randomly assigned into diabetic and rapamycin groups (n = 10). Ten healthy normal adult male rats of same age formed the control group. All groups were followed for 3 months. Rapamycin group received 1 mg/kg rapamycin via orogastric gavage during the last 4 weeks. At the end of 12 weeks, rats were sacrificed and biochemical oxidative stress markers (malondialdehyde and nitrotyrosine), together with vascular endothelial growth factor, hypoxia-inducible factor-1α, and pigment epithelium-derived factor, were measured in the retina. Blood biochemical analyses were also done. RESULTS: In the diabetic group, retinal malondialdehyde and nitrotyrosine levels were increased in comparison with control and rapamycin groups (p < 0.05). Rapamycin suppressed oxidative stress and showed a beneficial effect. It also decreased all angiomodulator cytokines compared with the diabetic group (p < 0.05). Correspondingly, rapamycin also decreased plasma malondialdehyde levels compared with the diabetic group (p = 0.037). CONCLUSIONS: Rapamycin may have a protective role against diabetes-induced oxidative retinal injury and may decrease angiomodulator cytokines.
OBJECTIVE: To evaluate the role of rapamycin in the prevention of diabetic oxidative stress and the regulation of angiogenic factors. DESIGN: Experimental animal study. METHODS:Diabetes was induced in 20 adult male Wistar rats by a single intraperitoneal administration of streptozotocin (60 mg/kg). Rats were randomly assigned into diabetic and rapamycin groups (n = 10). Ten healthy normal adult male rats of same age formed the control group. All groups were followed for 3 months. Rapamycin group received 1 mg/kg rapamycin via orogastric gavage during the last 4 weeks. At the end of 12 weeks, rats were sacrificed and biochemical oxidative stress markers (malondialdehyde and nitrotyrosine), together with vascular endothelial growth factor, hypoxia-inducible factor-1α, and pigment epithelium-derived factor, were measured in the retina. Blood biochemical analyses were also done. RESULTS: In the diabetic group, retinal malondialdehyde and nitrotyrosine levels were increased in comparison with control and rapamycin groups (p < 0.05). Rapamycin suppressed oxidative stress and showed a beneficial effect. It also decreased all angiomodulator cytokines compared with the diabetic group (p < 0.05). Correspondingly, rapamycin also decreased plasma malondialdehyde levels compared with the diabetic group (p = 0.037). CONCLUSIONS:Rapamycin may have a protective role against diabetes-induced oxidative retinal injury and may decrease angiomodulator cytokines.
Authors: Dongxu Fu; Jeremy Y Yu; Shihe Yang; Mingyuan Wu; Samar M Hammad; Anna R Connell; Mei Du; Junping Chen; Timothy J Lyons Journal: Diabetologia Date: 2016-07-30 Impact factor: 10.122
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