Literature DB >> 25283807

Enhanced cellular content and lactate fraction of the poly(lactate-co-3-hydroxybutyrate) polyester produced in recombinant Escherichia coli by the deletion of σ factor RpoN.

Ryosuke Kadoya1, Yu Kodama2, Ken'ichiro Matsumoto2, Seiichi Taguchi3.   

Abstract

A new approach at the transcriptional level was applied to lactate-based polyester production. Four σ factor disruptants, ΔrpoN, ΔrpoS, ΔfliA and ΔfecI, of Escherichia coli were used as hosts for poly(lactate-co-3-hydroxybutyrate) production from glucose. Among them, ΔrpoN caused dual positive effects of polymer production, enhanced cellular content and lactate fraction.
Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

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Keywords:  Biobased plastic; Poly(lactic acid); Polyhydroxyalkanoate; Polyhydroxybutyrate; σ factor

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Year:  2014        PMID： 25283807     DOI： 10.1016/j.jbiosc.2014.09.001

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  1 in total

1.  MtgA Deletion-Triggered Cell Enlargement of Escherichia coli for Enhanced Intracellular Polyester Accumulation.

Authors:  Ryosuke Kadoya; Ken'ichiro Matsumoto; Toshihiko Ooi; Seiichi Taguchi
Journal:  PLoS One       Date:  2015-06-03       Impact factor: 3.240
  1 in total

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