M Feres1, Mac Bernal1, F Matarazzo2, M Faveri1, P M Duarte1, L C Figueiredo1. 1. Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil. 2. Department of Periodontology, State University of Maringá, Maringá, Paraná, Brazil.
Abstract
BACKGROUND: The aim of this study was to compare subgingival bacterial recolonization patterns after scaling and root planing in current smokers and non-smokers. METHODS: 15 smokers and 15 non-smokers with chronic periodontitis received scaling and root planing in six visits lasting one hour each, over a period of 21 days. Clinical monitoring was performed at baseline and 180 days, and microbiological monitoring was performed at baseline, immediately after scaling and root planing (Day 0) and at 42, 63 and 180 days post-therapy. Subgingival plaque samples were analysed by checkerboard DNA-DNA hybridization. RESULTS: An improvement in clinical condition was observed for smokers and non-smokers; however, non-smokers showed a greater reduction in mean clinical attachment level in intermediate sites in comparison with smokers (p < 0.05). At Day 0, there was a significant reduction in the mean counts of the three pathogens from the red complex, Eubacterium nodatum and Parvimonas micra only in non-smokers (p < 0.05). There was a significant increase in the proportion of host-compatible species in non-smokers and smokers from baseline to 180 days post-therapy (p < 0.05). However, a significant decrease in the pathogenic species was observed only in non-smokers. CONCLUSIONS: Smokers were more susceptible to the re-establishment of a pathogenic subgingival biofilm than non-smokers.
BACKGROUND: The aim of this study was to compare subgingival bacterial recolonization patterns after scaling and root planing in current smokers and non-smokers. METHODS: 15 smokers and 15 non-smokers with chronic periodontitis received scaling and root planing in six visits lasting one hour each, over a period of 21 days. Clinical monitoring was performed at baseline and 180 days, and microbiological monitoring was performed at baseline, immediately after scaling and root planing (Day 0) and at 42, 63 and 180 days post-therapy. Subgingival plaque samples were analysed by checkerboard DNA-DNA hybridization. RESULTS: An improvement in clinical condition was observed for smokers and non-smokers; however, non-smokers showed a greater reduction in mean clinical attachment level in intermediate sites in comparison with smokers (p < 0.05). At Day 0, there was a significant reduction in the mean counts of the three pathogens from the red complex, Eubacterium nodatum and Parvimonas micra only in non-smokers (p < 0.05). There was a significant increase in the proportion of host-compatible species in non-smokers and smokers from baseline to 180 days post-therapy (p < 0.05). However, a significant decrease in the pathogenic species was observed only in non-smokers. CONCLUSIONS: Smokers were more susceptible to the re-establishment of a pathogenic subgingival biofilm than non-smokers.
Authors: Iris Zeller; Marina V Malovichko; Harrell E Hurst; Diane E Renaud; David A Scott Journal: J Periodontal Res Date: 2019-04-14 Impact factor: 4.419
Authors: Daniel Hagenfeld; Johannes Matern; Karola Prior; Inga Harks; Peter Eickholz; Katrin Lorenz; Ti-Sun Kim; Thomas Kocher; Jörg Meyle; Doğan Kaner; Ulrich Schlagenhauf; Dag Harmsen; Benjamin Ehmke Journal: Front Cell Infect Microbiol Date: 2020-05-05 Impact factor: 5.293