J Xu1, B Li1, Q Liu1, Y Shi1, J Peng1, M Jia1, Yan Liu2. 1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China. 2. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China. chblyan@163.com.
Abstract
BACKGROUND: Cryopreservation has been proved to be an efficient method for the long-term storage of pollen, based on our previous studies establishing cryo pollen banks for Camellia, Paeonia, and Prunus mume. OBJECTIVE: In the present study, we aimed to extend and verify the applicability of the cryopreservation protocol for ornamental plants. MATERIALS AND METHODS: 71 species or cultivars from 19 families were cryopreserved using the established protocol. RESULTS: Pollen from 60 species or cultivars (85% of those experimented on) retained viability after 1 year's cryopreservation; of these, 36 (51% of the total investigated) retained a sufficiently high germination capability compared with fresh pollen to enable longer term cryopreservation. CONCLUSION: This study confirms that wide-scale pollen banking of ornamental plants is feasible.
BACKGROUND: Cryopreservation has been proved to be an efficient method for the long-term storage of pollen, based on our previous studies establishing cryo pollen banks for Camellia, Paeonia, and Prunus mume. OBJECTIVE: In the present study, we aimed to extend and verify the applicability of the cryopreservation protocol for ornamental plants. MATERIALS AND METHODS: 71 species or cultivars from 19 families were cryopreserved using the established protocol. RESULTS: Pollen from 60 species or cultivars (85% of those experimented on) retained viability after 1 year's cryopreservation; of these, 36 (51% of the total investigated) retained a sufficiently high germination capability compared with fresh pollen to enable longer term cryopreservation. CONCLUSION: This study confirms that wide-scale pollen banking of ornamental plants is feasible.
Authors: Min-Rui Wang; Long Chen; Jaime A Teixeira da Silva; Gayle M Volk; Qiao-Chun Wang Journal: Plant Cell Rep Date: 2018-01-11 Impact factor: 4.570