Yupan Zhang1, Guoyue Lv2, Xiaoqian Lou3, Di Peng4, Xiaozhang Qu5, Xige Yang6, Desalegn Admassu Ayana7, Hui Guo8, Yanfang Jiang9. 1. Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China. Electronic address: zhangyupan007@163.com. 2. Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China. Electronic address: lgy08@sina.com. 3. Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China. Electronic address: xiaolou001009@163.com. 4. Department of Endocrinology, Center Hospital of Tonghua City, Tonghua 134000, China. Electronic address: pengdi55@126.com. 5. Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China. Electronic address: quxiaozhang2013@163.com. 6. Department of Anesthesiology, The First Hospital, Jilin University, Changchun 130021, China. Electronic address: xige_yang@163.com. 7. Department of Medical Laboratory Sciences, Haramaya University, Dire Dawa, Ethiopia. Electronic address: desadmassu@gmail.com. 8. Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China. Electronic address: nfmguohui@126.com. 9. Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: yanfangjiang@hotmail.com.
Abstract
BACKGROUND: Graves' disease (GD) is an organ-specific autoimmune disease. A significant decrease of the distribution of NK cells in the peripheral blood in children and adolescents with untreated GD has been observed. However, the role of NK and its subsets in adults with GD remains unclear. METHODS: A total of 28 adult patients with new onset of GD and 23 healthy controls (HC) were recruited. The number of activated inhibitory NK cells in peripheral blood of individual subjects was determined by flow cytometry. RESULTS: The number of CD3(-)CD56(+) and CD3(-)CD16(+)NK cells in peripheral blood was significantly decreased in the GD patients than the HC. Compared to the HCs, decreased number of NKG2D(+), NKG2C(+), NKp30(+) and NKG2A(+) NK cells and increased number of KIR3DL1(+) NK cells were detected in the GD patients. Moreover, the number of inducible CD107a(+) and IFN-γ-secreting NK cells in GD patients significantly decreased than those in HC. Interestingly, the number of NKG2A(+)NK cells was negatively correlated with the level of serum TRAb in GD patients. CONCLUSION: Our data indicate that decreased number and impaired function of NK cells may contribute to the pathogenesis of GD.
BACKGROUND:Graves' disease (GD) is an organ-specific autoimmune disease. A significant decrease of the distribution of NK cells in the peripheral blood in children and adolescents with untreated GD has been observed. However, the role of NK and its subsets in adults with GD remains unclear. METHODS: A total of 28 adult patients with new onset of GD and 23 healthy controls (HC) were recruited. The number of activated inhibitory NK cells in peripheral blood of individual subjects was determined by flow cytometry. RESULTS: The number of CD3(-)CD56(+) and CD3(-)CD16(+)NK cells in peripheral blood was significantly decreased in the GDpatients than the HC. Compared to the HCs, decreased number of NKG2D(+), NKG2C(+), NKp30(+) and NKG2A(+) NK cells and increased number of KIR3DL1(+) NK cells were detected in the GDpatients. Moreover, the number of inducible CD107a(+) and IFN-γ-secreting NK cells in GDpatients significantly decreased than those in HC. Interestingly, the number of NKG2A(+)NK cells was negatively correlated with the level of serum TRAb in GDpatients. CONCLUSION: Our data indicate that decreased number and impaired function of NK cells may contribute to the pathogenesis of GD.
Authors: Todd Bradley; Dimitra Peppa; Isabela Pedroza-Pacheco; Dapeng Li; Derek W Cain; Ricardo Henao; Vaishnavi Venkat; Bhavna Hora; Yue Chen; Nathan A Vandergrift; R Glenn Overman; R Whitney Edwards; Chris W Woods; Georgia D Tomaras; Guido Ferrari; Geoffrey S Ginsburg; Mark Connors; Myron S Cohen; M Anthony Moody; Persephone Borrow; Barton F Haynes Journal: Cell Date: 2018-09-27 Impact factor: 41.582
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