Ruqaiyyah Siddiqui1, Sahreena Lakhundi1, Naveed Ahmed Khan2. 1. Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan. 2. Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan. Electronic address: naveed5438@gmail.com.
Abstract
PURPOSE: The aim of this study was to assess the antimicrobial effects of marketed contact lens disinfecting solutions. METHODS: Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal, fungicidal and amoebicidal assays of eight different contact lens solutions including: ReNu MultiPlus, DuraPlus, Ultimate Plus, OptiFree Express, Kontex Clean, Kontex Normal, Kontex Multisol extra(+), Kontex Soak were performed. The efficacy of contact lens solutions was determined against keratitis-causing microbes, namely: Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Fusarium solani and Acanthamoeba castellanii. RESULTS: The results revealed that ReNu MultiPlus, DuraPlus and OptiFree Express were effective in killing bacterial and fungal pathogens as per manufacturer's minimum recommended disinfection time. Ultimate Plus was effective against F. solani and MRSA but ineffective against P. aeruginosa, S. marcescens and S. aureus. Of concern however, is that none of the locally formulated contact lens disinfecting solutions from Pakistan, i.e., Kontex Clean, Kontex Normal, Kontex Multisol extra(+) and Kontex Soak were effective against any of the keratitis-causing organisms tested. All eight contact lens disinfecting solutions were unable to destroy Acanthamoeba cysts. CONCLUSIONS: Because such ineffective contact lens disinfection solutions present a major risk to public health, these findings are of great concern to the health officials and to the manufacturers of the contact lens disinfection solutions and effective solutions are needed, along with emphasis on proper hygiene for contact lens care and special guidelines for developing countries regarding the manufacture and storage of contact lens disinfecting solutions.
PURPOSE: The aim of this study was to assess the antimicrobial effects of marketed contact lens disinfecting solutions. METHODS: Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal, fungicidal and amoebicidal assays of eight different contact lens solutions including: ReNu MultiPlus, DuraPlus, Ultimate Plus, OptiFree Express, Kontex Clean, Kontex Normal, Kontex Multisol extra(+), Kontex Soak were performed. The efficacy of contact lens solutions was determined against keratitis-causing microbes, namely: Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Fusarium solani and Acanthamoeba castellanii. RESULTS: The results revealed that ReNu MultiPlus, DuraPlus and OptiFree Express were effective in killing bacterial and fungal pathogens as per manufacturer's minimum recommended disinfection time. Ultimate Plus was effective against F. solani and MRSA but ineffective against P. aeruginosa, S. marcescens and S. aureus. Of concern however, is that none of the locally formulated contact lens disinfecting solutions from Pakistan, i.e., Kontex Clean, Kontex Normal, Kontex Multisol extra(+) and Kontex Soak were effective against any of the keratitis-causing organisms tested. All eight contact lens disinfecting solutions were unable to destroy Acanthamoeba cysts. CONCLUSIONS: Because such ineffective contact lens disinfection solutions present a major risk to public health, these findings are of great concern to the health officials and to the manufacturers of the contact lens disinfection solutions and effective solutions are needed, along with emphasis on proper hygiene for contact lens care and special guidelines for developing countries regarding the manufacture and storage of contact lens disinfecting solutions.
Authors: Grit Walther; Serena Stasch; Kerstin Kaerger; Axel Hamprecht; Mathias Roth; Oliver A Cornely; Gerd Geerling; Colin R Mackenzie; Oliver Kurzai; Marie von Lilienfeld-Toal Journal: J Clin Microbiol Date: 2017-07-26 Impact factor: 5.948
Authors: Rosnani Hanim Mohd Hussain; Wan Nur Afiqah; Mohamed Kamel Abdul Ghani; Naveed Ahmed Khan; Ruqaiyyah Siddiqui; Tengku Shahrul Anuar Journal: Saudi J Biol Sci Date: 2021-01-26 Impact factor: 4.219