Georgios Antonios Margonis1, Nikolaos Christoloukas2, Efstathios Antoniou3, Nikolaos Arkadopoulos4, George Theodoropoulos2, George Agrogiannis5, Emmanouil Pikoulis6, Efstratios S Patsouris5, George C Zografos2, Apostolos E Papalois7. 1. 1st Department of Propaedeutic Surgery, Hippokrateio Hospital, School of Medicine, University of Athens, Athens, Greece. Electronic address: antonis.margonis@gmail.com. 2. 1st Department of Propaedeutic Surgery, Hippokrateio Hospital, School of Medicine, University of Athens, Athens, Greece. 3. 2nd Department of Propaedeutic Surgery, Laiko Hospital, School of Medicine, University of Athens, Athens, Greece. 4. 4th Department of Surgery, Attikon University Hospital, School of Medicine, University of Athens, Athens, Greece. 5. 1st Department of Pathology, School of Medicine, University of Athens, Athens, Greece. 6. 1st Department of Surgery, Laiko Hospital, School of Medicine, University of Athens, Athens, Greece. 7. Experimental-Research Center, Elpen Pharmaceuticals, Athens, Greece.
Abstract
BACKGROUND: Crohn disease is still incurable. Compounds with anti-inflammatory and/or antioxidative effects are tested in various preclinical models of the disease. Our aim was to investigate the effects of sildenafil and lazaroid U-74389G in an experimental rat model of trinitrobenzenesulfonic acid-induced colitis. MATERIALS AND METHODS: Trinitrobenzenesulfonic acid was instilled into the colon of all male Wistar rats except for the rats belonging to the first group. For 6 days, the animals in group 3 were administered daily sildenafil orally, the rats in group 4 were administered daily U-74389G intravenously, and the rats in group 5 were coadministered daily sildenafil orally and intravenous U-74389G. The rats in groups 1 and 2 were not administered any treatment. During the study, the weights were recorded as a marker of clinical condition. The colon damage was evaluated using macroscopic colon mucosal damage index (CMDI), microscopic (Geboes score), and biochemical methods (tissue tumor necrosis factor [TNF]-α and malondialdehyde [MDA]). RESULTS: Sildenafil reduced TNF-α tissue levels and increased body weight. U-74389G reduced TNF-α, the macroscopic index of mucosal damage score (CMDI) and increased body weight. The combined treatment with sildenafil and U-74389G reduced tissue levels of both TNF-α and MDA, lowered CMDI and microscopic Geboes score, and increased body weight. CONCLUSIONS: U-74389G demonstrated a significant anti-inflammatory activity related to its ability to reduce colonic TNF-α, CMDI score, and improve weight change. We confirmed that sildenafil has anti-inflammatory capacity by reducing colonic TNF-α and by improving body weight. Finally, the combined treatment showed superior effects by reducing colonic TNF-α, colonic MDA, CMDI score, Geboes score, and by improving weight.
BACKGROUND:Crohn disease is still incurable. Compounds with anti-inflammatory and/or antioxidative effects are tested in various preclinical models of the disease. Our aim was to investigate the effects of sildenafil and lazaroid U-74389G in an experimental rat model of trinitrobenzenesulfonic acid-induced colitis. MATERIALS AND METHODS:Trinitrobenzenesulfonic acid was instilled into the colon of all male Wistar rats except for the rats belonging to the first group. For 6 days, the animals in group 3 were administered daily sildenafil orally, the rats in group 4 were administered daily U-74389G intravenously, and the rats in group 5 were coadministered daily sildenafil orally and intravenous U-74389G. The rats in groups 1 and 2 were not administered any treatment. During the study, the weights were recorded as a marker of clinical condition. The colon damage was evaluated using macroscopic colon mucosal damage index (CMDI), microscopic (Geboes score), and biochemical methods (tissue tumor necrosis factor [TNF]-α and malondialdehyde [MDA]). RESULTS:Sildenafil reduced TNF-α tissue levels and increased body weight. U-74389G reduced TNF-α, the macroscopic index of mucosal damage score (CMDI) and increased body weight. The combined treatment with sildenafil and U-74389G reduced tissue levels of both TNF-α and MDA, lowered CMDI and microscopic Geboes score, and increased body weight. CONCLUSIONS: U-74389G demonstrated a significant anti-inflammatory activity related to its ability to reduce colonic TNF-α, CMDI score, and improve weight change. We confirmed that sildenafil has anti-inflammatory capacity by reducing colonic TNF-α and by improving body weight. Finally, the combined treatment showed superior effects by reducing colonic TNF-α, colonic MDA, CMDI score, Geboes score, and by improving weight.
Authors: Maria Chalasti; Christos Iordanou; Zisis Kratiras; Aikaterini Stylianaki; Eleni-Andriana Trigka; Eleftheria Lakiotaki; Kali Makedou; Stavros Iliadis; Konstantinos G Zografos; Dimitrios Dimitroulis; Michail Chrisofos; Efstratios Patsouris; Georgios C Zografos; George C Bouboulis; Apostolos E Papalois Journal: J Int Med Res Date: 2020-06 Impact factor: 1.671
Authors: Apostolos E Papalois; Calypso Barbatis; Dimosthenis Chrysikos; Maria Korontzi; Michail Sideris; Theodoros Pittaras; Eleni Triantafyllidi; Alexandros Nomikos; John K Triantafillidis Journal: Biomed Res Int Date: 2019-10-09 Impact factor: 3.411