BACKGROUND: Soft tissue regeneration and remodeling is fundamental in periodontal surgery, thus we investigated the angiogenic response elicited in the subcutaneous tissue of rats by a proprietary, polyethylene glycol hydrogel formulation (PEG) alone or conjugated with specific amelogenins (EMD) or nanobioglass particles (NBG). METHODS: Discs with three different formulations (PEG, PEG-EMD, and PEG-NBG) were inserted into four unconnected subcutaneous pouches, produced on the back of Sprague-Dawley rats (n=56, divided into three groups), and used for blood flow evaluation by Laser Doppler analysis at 1, 2, 4, 8, and 16 weeks or for histological and immunohistochemical analysis at 1, 2, 4, 8, and 16 weeks. RESULTS: All formulations showed tissue integration, absence of inflammatory reaction (as revealed by myeloperoxidase staining), and increased vascularization (by counting microvascular density following CD31 staining). Laser Doppler analysis revealed a statistically significant increase in blood flow after 1 week for PEG-EMD and after 2 weeks for PEG-NBG. The angiogenic response was significantly increased at 1, 2, and 8 weeks for PEG-EMD, but only at 4 weeks for PEG-NBG. CONCLUSIONS: The studied biomaterials revealed equal biocompatibility and tissue integration properties. PEG-EMD showed the most pronounced and consistent angiogenic response in the early phases of wound healing, while the PEG-NBG formulation provided a slower and delayed, but relevant, response.
BACKGROUND: Soft tissue regeneration and remodeling is fundamental in periodontal surgery, thus we investigated the angiogenic response elicited in the subcutaneous tissue of rats by a proprietary, polyethylene glycol hydrogel formulation (PEG) alone or conjugated with specific amelogenins (EMD) or nanobioglass particles (NBG). METHODS: Discs with three different formulations (PEG, PEG-EMD, and PEG-NBG) were inserted into four unconnected subcutaneous pouches, produced on the back of Sprague-Dawley rats (n=56, divided into three groups), and used for blood flow evaluation by Laser Doppler analysis at 1, 2, 4, 8, and 16 weeks or for histological and immunohistochemical analysis at 1, 2, 4, 8, and 16 weeks. RESULTS: All formulations showed tissue integration, absence of inflammatory reaction (as revealed by myeloperoxidase staining), and increased vascularization (by counting microvascular density following CD31 staining). Laser Doppler analysis revealed a statistically significant increase in blood flow after 1 week for PEG-EMD and after 2 weeks for PEG-NBG. The angiogenic response was significantly increased at 1, 2, and 8 weeks for PEG-EMD, but only at 4 weeks for PEG-NBG. CONCLUSIONS: The studied biomaterials revealed equal biocompatibility and tissue integration properties. PEG-EMD showed the most pronounced and consistent angiogenic response in the early phases of wound healing, while the PEG-NBG formulation provided a slower and delayed, but relevant, response.
Authors: Ronald E Jung; Roger Zwahlen; Franz E Weber; Aart Molenberg; G Harry van Lenthe; Christoph H F Hammerle Journal: Clin Oral Implants Res Date: 2006-08 Impact factor: 5.977
Authors: Monika Herten; Ronald E Jung; Daniel Ferrari; Daniel Rothamel; Vladimir Golubovic; Aart Molenberg; Christoph H F Hämmerle; Jürgen Becker; Frank Schwarz Journal: Clin Oral Implants Res Date: 2008-12-01 Impact factor: 5.977
Authors: Ronald E Jung; Christoph H Hämmerle; Vladimir Kokovic; Franz E Weber Journal: Int J Oral Maxillofac Implants Date: 2007 Mar-Apr Impact factor: 2.804
Authors: Sandra Wechsler; Daniel Fehr; Aart Molenberg; George Raeber; Jason C Schense; Franz E Weber Journal: J Biomed Mater Res A Date: 2008-05 Impact factor: 4.396
Authors: João César Zielak; David Gulin Neto; Makeli Aparecida Cazella Zielak; Leonardo Brunet Savaris; Fernando Luis Esteban Florez; Tatiana Miranda Deliberador Journal: Heliyon Date: 2018-09-14