Jun Muto1, Ken Shirabe2, Tomoharu Yoshizumi3, Toru Ikegami3, Shinichi Aishima4, Kousei Ishigami5, Yoshikazu Yonemitsu6, Tetsuo Ikeda3, Yuji Soejima3, Yoshihiko Maehara3. 1. Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan R&D Laboratory for Innovative Biotherapeutics, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan. 2. Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan kshirabe@surg2.med.kyushu-u.ac.jp. 3. Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. 4. Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. 5. Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. 6. R&D Laboratory for Innovative Biotherapeutics, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Abstract
AIM: The apelin-APJ system regulates angiogenesis, and is overexpressed in several types of cancer. The aim of this study was to clarify the role of the apelin-APJ system in the angiogenesis of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Expressions of angiogenic factors and vascular markers were investigated in specimens from 90 HCC patients. A subcutaneous HCC tumor mouse model was treated with the APJ antagonist, F13A, and tumor growth and vascular development were assessed. RESULTS: APJ expression was observed in arteriole-smooth muscle. Higher amounts of APJ(+)-arteriole and apelin were detected in tumors (p<0.001 for both). APJ(+)-arteriole and apelin expression were more commonly observed in moderately- and poorly-differentiated than in well-differentiated HCC (p ≤ 0.003). HCC with irregular dilated arteries expressed higher levels of apelin (p=0.012). Tumor growth was inhibited by treatment with F13A (p<0.001), and arterioles were decreased in the treated group (p=0.047), in vivo. CONCLUSION: Apelin-APJ is overexpressed, and works as a signal for arteriogenesis in HCC. Copyright
AIM: The apelin-APJ system regulates angiogenesis, and is overexpressed in several types of cancer. The aim of this study was to clarify the role of the apelin-APJ system in the angiogenesis of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Expressions of angiogenic factors and vascular markers were investigated in specimens from 90 HCC patients. A subcutaneous HCC tumormouse model was treated with the APJ antagonist, F13A, and tumor growth and vascular development were assessed. RESULTS:APJ expression was observed in arteriole-smooth muscle. Higher amounts of APJ(+)-arteriole and apelin were detected in tumors (p<0.001 for both). APJ(+)-arteriole and apelin expression were more commonly observed in moderately- and poorly-differentiated than in well-differentiated HCC (p ≤ 0.003). HCC with irregular dilated arteries expressed higher levels of apelin (p=0.012). Tumor growth was inhibited by treatment with F13A (p<0.001), and arterioles were decreased in the treated group (p=0.047), in vivo. CONCLUSION: Apelin-APJ is overexpressed, and works as a signal for arteriogenesis in HCC. Copyright
Authors: Chad Hall; Laurent Ehrlich; Julie Venter; April O'Brien; Tori White; Tianhao Zhou; Tien Dang; Fanyin Meng; Pietro Invernizzi; Francesca Bernuzzi; Gianfranco Alpini; Terry C Lairmore; Shannon Glaser Journal: Cancer Lett Date: 2016-11-26 Impact factor: 8.679
Authors: Huarong Chen; Chi-Chun Wong; Dabin Liu; Minnie Y Y Go; Bin Wu; Sui Peng; Ming Kuang; Nathalie Wong; Jun Yu Journal: Theranostics Date: 2019-07-09 Impact factor: 11.556
Authors: Danielle McAnally; Khandaker Siddiquee; Ahmed Gomaa; Andras Szabo; Stefan Vasile; Patrick R Maloney; Daniela B Divlianska; Satyamaheshwar Peddibhotla; Camilo J Morfa; Paul Hershberger; Rebecca Falter; Robert Williamson; David B Terry; Rafal Farjo; Anthony B Pinkerton; Xiaping Qi; Judith Quigley; Michael E Boulton; Maria B Grant; Layton H Smith Journal: PLoS One Date: 2018-09-12 Impact factor: 3.240