Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12.

The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983, J. Virol. 46, 454-465) have been tested for their ability to support Ad40 replication. Only cell lines which supply E1B functions, but not those expressing E1A alone, are permissive for Ad40, suggesting that Ad40 may require some function supplied by E1B or induced in E1B-containing cells. In coinfection assays Ad40 complements Ad5 dl312 (delta E1A) but not Ad5 dl313 (delta E1B) and is itself complemented by dl312 but not by dl313. Mutants of Ad2 and Ad12 with lesions in E1B 55K or 19K protein have been used to further delineate the requirements for Ad40 growth in HeLa cells. For mutants lacking 55K function there is minimal complementation in either direction, whereas those lacking only the 19K product are able to complement Ad40.