Joon Ha Park1, Ok Kyu Park2, Bingchun Yan3, Ji Hyeon Ahn1, In Hye Kim1, Jae-Chul Lee1, Seung-Hae Kwon2, Ki-Yeon Yoo4, Choong Hyun Lee5, In Koo Hwang6, Jung Hoon Choi7, Moo-Ho Won8, Jong-Dai Kim9. 1. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. 2. Division of Analytical Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, South Korea. 3. Department of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, China. 4. Department of Oral Anatomy, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, South Korea. 5. Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 330-714, South Korea. 6. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea. 7. Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea. 8. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Email: mhwon@kangwon.ac.kr. 9. Division of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, South Korea. Email: jongdai@cc.kangwon.ac.kr.
Abstract
BACKGROUND: Danshen (Radix Salvia miltiorrhizae) has been used as a traditional medicine in Asia for treatment of various microcirculatory disturbance related diseases. Tanshinones are mainly hydrophobic active components, which have been isolated from Danshen and show various biological functions. In this study, we observed the neuroprotective effect of tanshinone I (TsI) against ischemic damage in the gerbil hippocampal CA1 region (CA1) after transient cerebral ischemia and examined its neuroprotective mechanism. METHODS: The gerbils were divided into vehicle-treated-sham-group, vehicle-treated-ischemia-group, TsI-treated-sham-group, and TsI-treated-ischemia-group. TsI was administrated intraperitoneally three times (once a day for three days) before ischemia-reperfusion. The neuroprotective effect of TsI was examined using H&E staining, neuronal nuclei (NeuN) immunohistochemistry and Fluoro-Jade B staining. To investigate the neuroprotective mechanism of TsI after ischemia-reperfusion, immunohistochemical (IHC) and Western blotting analyses for Cu, Zn-superoxide dismutase (SOD1), Mn-superoxide dismutase (SOD2), brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-I (IGF-I) were performed. RESULTS: Treatment with TsI protected pyramidal neurons from ischemia-induced neuronal death in the CA1 after ischemia-reperfusion. In addition, treatment with TsI maintained the levels of SOD1 and SOD2 as determined by IHC and Western blotting in the CA1 after ischemia-reperfusion compared with the vehicle-ischemia-group. In addition, treatment with TsI increased the levels of BDNF and IGF-I determined by IHC and Western blotting in the TsI-treated-sham-group compared with the vehicle-treated-sham-group, and their levels were maintained in the stratum pyramidale of the ischemic CA1 in the TsI-treated-ischemia-group. CONCLUSION: Treatment with TsI protects pyramidal neurons of the CA1 from ischemic damage induced by transient cerebral ischemia via the maintenance of antioxidants and the increase of neurotrophic factors.
BACKGROUND: Danshen (Radix Salvia miltiorrhizae) has been used as a traditional medicine in Asia for treatment of various microcirculatory disturbance related diseases. Tanshinones are mainly hydrophobic active components, which have been isolated from Danshen and show various biological functions. In this study, we observed the neuroprotective effect of tanshinone I (TsI) against ischemic damage in the gerbil hippocampal CA1 region (CA1) after transient cerebral ischemia and examined its neuroprotective mechanism. METHODS: The gerbils were divided into vehicle-treated-sham-group, vehicle-treated-ischemia-group, TsI-treated-sham-group, and TsI-treated-ischemia-group. TsI was administrated intraperitoneally three times (once a day for three days) before ischemia-reperfusion. The neuroprotective effect of TsI was examined using H&E staining, neuronal nuclei (NeuN) immunohistochemistry and Fluoro-Jade B staining. To investigate the neuroprotective mechanism of TsI after ischemia-reperfusion, immunohistochemical (IHC) and Western blotting analyses for Cu, Zn-superoxide dismutase (SOD1), Mn-superoxide dismutase (SOD2), brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-I (IGF-I) were performed. RESULTS: Treatment with TsI protected pyramidal neurons from ischemia-induced neuronal death in the CA1 after ischemia-reperfusion. In addition, treatment with TsI maintained the levels of SOD1 and SOD2 as determined by IHC and Western blotting in the CA1 after ischemia-reperfusion compared with the vehicle-ischemia-group. In addition, treatment with TsI increased the levels of BDNF and IGF-I determined by IHC and Western blotting in the TsI-treated-sham-group compared with the vehicle-treated-sham-group, and their levels were maintained in the stratum pyramidale of the ischemicCA1 in the TsI-treated-ischemia-group. CONCLUSION: Treatment with TsI protects pyramidal neurons of the CA1 from ischemic damage induced by transient cerebral ischemia via the maintenance of antioxidants and the increase of neurotrophic factors.
Authors: Bai Hui Chen; Joon Ha Park; Jeong Hwi Cho; In Hye Kim; Jae Chul Lee; Tae-Kyeong Lee; Ji Hyeon Ahn; Hyun Jin Tae; Bich Na Shin; Jong-Dai Kim; Il Jun Kang; Moo-Ho Won; Yun Lyul Lee Journal: Neurochem Res Date: 2016-04-06 Impact factor: 3.996
Authors: Joon Ha Park; Tae-Kyeong Lee; Bing-Chun Yan; Bich-Na Shin; Ji Hyeon Ahn; In Hye Kim; Jeong Hwi Cho; Jae-Chul Lee; In Koo Hwang; Jong Dai Kim; Seongkweon Hong; Young Joo Lee; Moo-Ho Won; Il Jun Kang Journal: Chin Med J (Engl) Date: 2017-08-05 Impact factor: 2.628
Authors: In Hye Kim; Tae-Kyeong Lee; Jeong Hwi Cho; Jae-Chul Lee; Joon Ha Park; Ji Hyeon Ahn; Bich-Na Shin; Bai Hui Chen; Hyun-Jin Tae; Yang Hee Kim; Jong-Dai Kim; Young-Myeong Kim; Moo-Ho Won; Il Jun Kang Journal: Mol Med Rep Date: 2017-05-17 Impact factor: 2.952
Authors: Bai Hui Chen; Joon Ha Park; Ji Hyeon Ahn; Jeong Hwi Cho; In Hye Kim; Jae Chul Lee; Moo-Ho Won; Choong-Hyun Lee; In Koo Hwang; Jong-Dai Kim; Il Jun Kang; Jun Hwi Cho; Bich Na Shin; Yang Hee Kim; Yun Lyul Lee; Seung Min Park Journal: Neural Regen Res Date: 2017-02 Impact factor: 5.135
Authors: Tae-Kyeong Lee; Bai Hui Chen; Jae-Chul Lee; Myoung Cheol Shin; Jun Hwi Cho; Hyang-Ah Lee; Jung Hoon Choi; In Koo Hwang; Il Jun Kang; Ji Hyeon Ahn; Joon Ha Park; Soo Young Choi; Moo-Ho Won Journal: Mol Med Rep Date: 2018-04-13 Impact factor: 2.952
Authors: Ki-Yeon Yoo; In Hye Kim; Jeong-Hwi Cho; Ji Hyeon Ahn; Joon Ha Park; Jae-Chul Lee; Hyun-Jin Tae; Dae Won Kim; Jong-Dai Kim; Seongkweon Hong; Moo-Ho Won; Il Jun Kang Journal: Neural Regen Res Date: 2016-02 Impact factor: 5.135