| Literature DB >> 25267285 |
Fabio Ribeiro Braga1, Filippe Elias de Freitas Soares2, Juliana Milani Araujo3, Leandro Abreu da Fonseca4, Emy Hiura4, Maylla Garschagen Gava4, Fernanda Toledo Vieira4, Jeanne Saraiva da Paz4, Lorendane Milena de Carvalho3, João Victor Faccini3, José Humberto de Queiroz2, Jackson Victor Araújo3.
Abstract
The present work used Plackett-Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett-Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P < 0.05) on the destruction of S. westeri larvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P < 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P < 0.05) on the destruction of P. fastosum eggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.Entities:
Keywords: Biological control; Fungal enzymes; Helminths; Plackett–Burman
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Year: 2014 PMID： 25267285 DOI： 10.1016/j.rvsc.2014.09.005
Source DB: PubMed Journal: Res Vet Sci ISSN： 0034-5288 Impact factor: 2.534