Maria Teresa Arias-Moliz1, Ronald Ordinola-Zapata2, Pilar Baca3, Matilde Ruiz-Linares3, Carmen María Ferrer-Luque3. 1. Department of Microbiology, School of Dentistry, University of Granada, Spain. 2. Department of Endodontics, Bauru Dental School, University of São Paulo, Brazil. Electronic address: ronaldordinola@usp.br. 3. Department of Stomatology, School of Dentistry, University of Granada, Spain.
Abstract
INTRODUCTION: The aim of this study was to evaluate the antimicrobial activity of a 2.5% sodium hypochlorite (NaOCl)/9% etidronic acid (HEBP) irrigant solution on Enterococcus faecalis growing in biofilms and a dentinal tubule infection model. METHODS: The antimicrobial activity of the solutions 2.5% NaOCl and 9% HEBP alone and associated was evaluated on E. faecalis biofilms grown in the Calgary biofilm model (minimum biofilm eradication concentration high-throughput device). For the dentinal tubule infection test, the percentage of dead cells in E. faecalis-infected dentinal tubules treated with the solutions for 10 minutes was measured using confocal laser scanning microscopy and the live/dead technique. Available chlorine and pH of the solutions were also measured. Distilled water was used as the control. Nonparametric tests were used to determine statistical differences. RESULTS: The highest viability was found in the distilled water group and the lowest in the NaOCl-treated dentin (P < .05). Both NaOCl solutions killed 100% of the E. faecalis biofilms and showed the highest antimicrobial activity inside dentinal tubules, without statistical differences between the 2 (P < .05). The HEBP isolated solution killed bacteria inside dentinal tubules but did not present any significant effect against E. faecalis biofilms. The incorporation of HEBP to NaOCl did not cause any loss of available chlorine within 60 minutes. CONCLUSIONS: HEBP did not interfere with the ability of NaOCl to kill E. faecalis grown in biofilms and inside dentinal tubules.
INTRODUCTION: The aim of this study was to evaluate the antimicrobial activity of a 2.5% sodium hypochlorite (NaOCl)/9% etidronic acid (HEBP) irrigant solution on Enterococcus faecalis growing in biofilms and a dentinal tubule infection model. METHODS: The antimicrobial activity of the solutions 2.5% NaOCl and 9% HEBP alone and associated was evaluated on E. faecalis biofilms grown in the Calgary biofilm model (minimum biofilm eradication concentration high-throughput device). For the dentinal tubule infection test, the percentage of dead cells in E. faecalis-infected dentinal tubules treated with the solutions for 10 minutes was measured using confocal laser scanning microscopy and the live/dead technique. Available chlorine and pH of the solutions were also measured. Distilled water was used as the control. Nonparametric tests were used to determine statistical differences. RESULTS: The highest viability was found in the distilled water group and the lowest in the NaOCl-treated dentin (P < .05). Both NaOCl solutions killed 100% of the E. faecalis biofilms and showed the highest antimicrobial activity inside dentinal tubules, without statistical differences between the 2 (P < .05). The HEBP isolated solution killed bacteria inside dentinal tubules but did not present any significant effect against E. faecalis biofilms. The incorporation of HEBP to NaOCl did not cause any loss of available chlorine within 60 minutes. CONCLUSIONS:HEBP did not interfere with the ability of NaOCl to kill E. faecalis grown in biofilms and inside dentinal tubules.
Authors: Luciano Giardino; Massimo Del Fabbro; Marco Morra; Thais Pereira; Flaviana Bombarda de Andrade; Paolo Savadori; Luigi Generali Journal: Odontology Date: 2019-06-13 Impact factor: 2.634
Authors: Mariana Maciel Batista Borges; René J B Dijkstra; Flaviana Bombarda de Andrade; Marco Antonio Hungaro Duarte; Michel Versluis; Lucas W M van der Sluis; Xenos Petridis Journal: Int Endod J Date: 2022-05-06 Impact factor: 5.165