PROBLEM: Group B Streptococcus (GBS) is a leading cause of neonatal morbidity and mortality. We tested the hypothesis that the choriodecidua plays a role in GBS-stimulated human beta defensin(HBD)-2 increases in amnion cells through a secreted factor of choriodecidual origin. METHOD OF STUDY: Human amnion epithelial cells were treated with choriodecidual GBS-conditioned medium, live GBS, lipoteichoic acid (LTA), or lipopolysaccharide (LPS), with and without IL-1 inhibitors. RESULTS: Choriodecidual tissue punches released IL-1α and IL-1β in response to GBS and this medium significantly stimulated release of HBD-2 by amnion cell cultures. Inhibitors of IL-1 significantly impaired the release of HBD-2 from amnion cells treated with GBS choriodecidual conditioned medium. Direct stimulation of amnion cells with GBS, LTA, or LPS did not increase HBD-2 release. CONCLUSION: Paracrine signaling involving IL-1 of choriodecidual origin is likely a critical driver for amnion HBD-2 increases in response to GBS infection of extraplacental membranes.
PROBLEM: Group B Streptococcus (GBS) is a leading cause of neonatal morbidity and mortality. We tested the hypothesis that the choriodecidua plays a role in GBS-stimulated humanbeta defensin(HBD)-2 increases in amnion cells through a secreted factor of choriodecidual origin. METHOD OF STUDY: Human amnion epithelial cells were treated with choriodecidual GBS-conditioned medium, live GBS, lipoteichoic acid (LTA), or lipopolysaccharide (LPS), with and without IL-1 inhibitors. RESULTS: Choriodecidual tissue punches released IL-1α and IL-1β in response to GBS and this medium significantly stimulated release of HBD-2 by amnion cell cultures. Inhibitors of IL-1 significantly impaired the release of HBD-2 from amnion cells treated with GBS choriodecidual conditioned medium. Direct stimulation of amnion cells with GBS, LTA, or LPS did not increase HBD-2 release. CONCLUSION: Paracrine signaling involving IL-1 of choriodecidual origin is likely a critical driver for amnion HBD-2 increases in response to GBS infection of extraplacental membranes.
Authors: Glen C Ulett; Richard I Webb; Kimberly B Ulett; Xiangqin Cui; William H Benjamin; Michael Crowley; Mark A Schembri Journal: J Infect Dis Date: 2010-03-15 Impact factor: 5.226
Authors: C Iavazzo; K Tassis; D Gourgiotis; M Boutsikou; S Baka; D Hassiakos; A Hadjithomas; D Botsis; A Malamitsi-Puchner Journal: Arch Gynecol Obstet Date: 2009-06-25 Impact factor: 2.344
Authors: Hae-Ryung Park; Sean M Harris; Erica Boldenow; Richard C McEachin; Maureen Sartor; Mark Chames; Rita Loch-Caruso Journal: Biol Reprod Date: 2018-03-01 Impact factor: 4.285
Authors: Lisa M Rogers; Anjali P Anders; Ryan S Doster; Elizabeth A Gill; Juan S Gnecco; Jacob M Holley; Tara M Randis; Adam J Ratner; Jennifer A Gaddy; Kevin Osteen; David M Aronoff Journal: Am J Reprod Immunol Date: 2018-08-07 Impact factor: 3.886
Authors: Jessica A Sutton; Lisa M Rogers; Beverly R E A Dixon; Leslie Kirk; Ryan Doster; Holly M Algood; Jennifer A Gaddy; Rebecca Flaherty; Shannon D Manning; David M Aronoff Journal: Am J Reprod Immunol Date: 2019-01-30 Impact factor: 3.886