María Esther Rubio-Ruiz1, Israel Pérez-Torres2, Eulises Diaz-Diaz3, Natalia Pavón4, Verónica Guarner-Lans1. 1. Departments of Physiology, Instituto Nacional de Cardiología "Ignacio Chávez", Mexico City, Mexico. 2. Departments of Pathology, Instituto Nacional de Cardiología "Ignacio Chávez", Mexico City, Mexico. 3. Department of Reproductive Biology Instituto Nacional de Ciencias Médicas y de la Nutrición "Salvador Zubirán", Mexico City, Mexico. 4. Departments of Pharmacology, Instituto Nacional de Cardiología "Ignacio Chávez", Mexico City, Mexico.
Abstract
AIM: Metabolic syndrome (MS) and aging are low-grade systemic inflammatory conditions, and inflammation is a key component of endothelial dysfunction. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs (NSAIDs) upon the vascular reactivity in aging MS rats. METHODS: MS was induced in young male rats by adding 30% sucrose in drinking water over 6, 12, and 18 months. When the treatment was finished, the blood samples were collected, and aortas were dissected out. The expression of COX isoenzymes and PLA2 in the aortas was analyzed using Western blot analysis. The contractile responses of aortic rings to norepinephrine (1 μmol/L) were measured in the presence or absence of different NSAIDs (10 μmol/L for each). RESULTS: Serum levels of pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β) in control rats were remained stable during the aging process, whereas serum IL-6 in MS rats were significantly increased at 12 and 18 months. The levels of COX isoenzyme and PLA2 in aortas from control rats increased with the aging, whereas those in aortas from MS rats were irregularly increased with the highest levels at 6 months. Pretreatment with acetylsalicylic acid (a COX-1 preferential inhibitor), indomethacin (a non-selective COX inhibitor) or meloxicam (a COX-2 preferential inhibitor) decreased NE-induced contractions of aortic rings from MS rats at all the ages, with meloxicam being the most potent. Acetylsalicylic acid also significantly reduced the maximum responses of ACh-induced vasorelaxation of aortic rings from MS rats, but indomethacin and meloxicam had no effect. CONCLUSION: NSAIDs can directly affect vascular responses in aging MS rats. Understanding the effects of NSAIDs on blood vessels may improve the treatment of cardiovascular diseases and MS in the elders.
AIM: Metabolic syndrome (MS) and aging are low-grade systemic inflammatory conditions, and inflammation is a key component of endothelial dysfunction. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs (NSAIDs) upon the vascular reactivity in aging MS rats. METHODS: MS was induced in young male rats by adding 30% sucrose in drinking water over 6, 12, and 18 months. When the treatment was finished, the blood samples were collected, and aortas were dissected out. The expression of COX isoenzymes and PLA2 in the aortas was analyzed using Western blot analysis. The contractile responses of aortic rings to norepinephrine (1 μmol/L) were measured in the presence or absence of different NSAIDs (10 μmol/L for each). RESULTS: Serum levels of pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β) in control rats were remained stable during the aging process, whereas serum IL-6 in MS rats were significantly increased at 12 and 18 months. The levels of COX isoenzyme and PLA2 in aortas from control rats increased with the aging, whereas those in aortas from MS rats were irregularly increased with the highest levels at 6 months. Pretreatment with acetylsalicylic acid (a COX-1 preferential inhibitor), indomethacin (a non-selective COX inhibitor) or meloxicam (a COX-2 preferential inhibitor) decreased NE-induced contractions of aortic rings from MS rats at all the ages, with meloxicam being the most potent. Acetylsalicylic acid also significantly reduced the maximum responses of ACh-induced vasorelaxation of aortic rings from MS rats, but indomethacin and meloxicam had no effect. CONCLUSION: NSAIDs can directly affect vascular responses in aging MS rats. Understanding the effects of NSAIDs on blood vessels may improve the treatment of cardiovascular diseases and MS in the elders.
Authors: J K Hennan; J Huang; T D Barrett; E M Driscoll; D E Willens; A M Park; L J Crofford; B R Lucchesi Journal: Circulation Date: 2001-08-14 Impact factor: 29.690
Authors: Malinalli Brianza-Padilla; Roxana Carbó; Julio C Arana; Gonzalo Vázquez-Palacios; Martha A Ballinas-Verdugo; Guillermo C Cardoso-Saldaña; Adán G Palacio; Yaneli Juárez-Vicuña; Fausto Sánchez; Eduardo Martínez-Martínez; Fengyang Huang; Fausto Sánchez-Muñoz; Rafael Bojalil Journal: Biomed Res Int Date: 2016-11-24 Impact factor: 3.411
Authors: Betzabé Nieto-Lima; Agustina Cano-Martínez; María E Rubio-Ruiz; Israel Pérez-Torres; Verónica Guarner-Lans Journal: Front Physiol Date: 2018-01-24 Impact factor: 4.566