| Literature DB >> 25261306 |
Wai-Ming Lee1, Yin Chen, Wensheng Wang, Anne Mosser.
Abstract
HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.Entities:
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Year: 2015 PMID： 25261306 DOI： 10.1007/978-1-4939-1571-2_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745