Donglai Deng1, Hongye Yang1, Jingmei Guo1, Xiaohui Chen1, Weiping Zhang2, Cui Huang3. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. 2. Lanzhou Hospital of Stomatology, Lanzhou, China. 3. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: huangcui@whu.edu.cn.
Abstract
OBJECTIVES: To compare the effects of four commonly used artificial ageing methods (water storage, thermocycling, NaOCl storage and pH cycling) on the degradation of adhesive-dentine interfaces. METHODS: Fifty molars were sectioned parallel to the occlusal plane, polished and randomly divided into two adhesive groups: An etch-and-rinse adhesive Adper SingleBond 2 and a self-etch adhesive G-Bond. After the composite built up, the specimens from each adhesive group were sectioned into beams, which were then assigned to one of the following groups: Group 1 (control), 24h of water storage; Group 2, 6 months of water storage; Group 3, 10,000 runs of thermocycling; Group 4, 1h of 10% NaOCl storage; and Group 5, 15 runs of pH cycling. The microtensile bond strengths were then tested. The failure modes were classified with a stereomicroscope and representative interface was analyzed with a field-emission scanning electron microscopy (FESEM). Nanoleakage expression was evaluated through FESEM in the backscattered mode. RESULTS: The four artificial ageing methods decreased the bonding strength to nearly 50% and increased the nanoleakage expression of both adhesive systems compared with the control treatment. Adhesive failures were the predominant fracture modes in all groups. However, differences in detailed morphology were observed among the different groups. CONCLUSIONS: Water storage, thermocycling, NaOCl storage and pH cycling could obtain similar degradation effectiveness through appropriate parameter selection. Each in vitro artificial ageing method had its own mechanisms, characteristics and application scope for degrading the adhesive-dentin interfaces. CLINICAL SIGNIFICANCE: Water storage is simple, low-cost but time-consuming; thermocycling lacks of a standard agreement; NaOCl storage is time-saving but mainly degrades the organic phase; pH cycling can resemble cariogenic condition but needs further studies. Researchers focusing on bonding durability studies should be deliberate in selecting an appropriate ageing model based on the differences of test material, purpose and time.
OBJECTIVES: To compare the effects of four commonly used artificial ageing methods (water storage, thermocycling, NaOCl storage and pH cycling) on the degradation of adhesive-dentine interfaces. METHODS: Fifty molars were sectioned parallel to the occlusal plane, polished and randomly divided into two adhesive groups: An etch-and-rinse adhesive Adper SingleBond 2 and a self-etch adhesive G-Bond. After the composite built up, the specimens from each adhesive group were sectioned into beams, which were then assigned to one of the following groups: Group 1 (control), 24h of water storage; Group 2, 6 months of water storage; Group 3, 10,000 runs of thermocycling; Group 4, 1h of 10% NaOCl storage; and Group 5, 15 runs of pH cycling. The microtensile bond strengths were then tested. The failure modes were classified with a stereomicroscope and representative interface was analyzed with a field-emission scanning electron microscopy (FESEM). Nanoleakage expression was evaluated through FESEM in the backscattered mode. RESULTS: The four artificial ageing methods decreased the bonding strength to nearly 50% and increased the nanoleakage expression of both adhesive systems compared with the control treatment. Adhesive failures were the predominant fracture modes in all groups. However, differences in detailed morphology were observed among the different groups. CONCLUSIONS:Water storage, thermocycling, NaOCl storage and pH cycling could obtain similar degradation effectiveness through appropriate parameter selection. Each in vitro artificial ageing method had its own mechanisms, characteristics and application scope for degrading the adhesive-dentin interfaces. CLINICAL SIGNIFICANCE: Water storage is simple, low-cost but time-consuming; thermocycling lacks of a standard agreement; NaOCl storage is time-saving but mainly degrades the organic phase; pH cycling can resemble cariogenic condition but needs further studies. Researchers focusing on bonding durability studies should be deliberate in selecting an appropriate ageing model based on the differences of test material, purpose and time.
Authors: Valeria B Gotti; Américo B Correr; Steven H Lewis; Victor P Feitosa; Lourenço Correr-Sobrinho; Jeffrey W Stansbury Journal: Dent Mater Date: 2016-09-21 Impact factor: 5.304
Authors: Heleine M C Rêgo; Thaís S Alves; Eduardo Bresciani; Li-Na Niu; Franklin R Tay; César R Pucci Journal: Sci Rep Date: 2016-11-25 Impact factor: 4.379