Hanen Bouaziz-Ketata1, Ghada Ben Salah2, Hichem Ben Salah3, Rim Marrekchi4, Kamel Jamoussi4, Tahia Boudawara5, Faiza Fakhfekh2, Najiba Zeghal1. 1. Animal Physiology Laboratory, UR/11 ES70, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia. 2. Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax 3029, Tunisia. 3. Laboratory of Chemistry, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia. 4. Laboratory of Biochemistry, CHU Hedi Chaker, Sfax 3029, Tunisia. 5. Histopathology Laboratory, University of Sfax, CHU Habib Bourguiba, Sfax 3029, Tunisia.
Abstract
OBJECTIVE: The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity. METHODS: Male Wistar rats were randomly divided into three groups: a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta. RESULTS: NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells' damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above. CONCLUSION: The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.
OBJECTIVE: The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity. METHODS: Male Wistar rats were randomly divided into three groups: a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta. RESULTS:NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells' damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above. CONCLUSION: The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.
Authors: K Kramkowski; A Leszczynska; K Przyborowski; B Proniewski; N Marcinczyk; U Rykaczewska; D Jarmoc; E Chabielska; S Chlopicki Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2016-10-14 Impact factor: 3.000