| Literature DB >> 25256503 |
Yuan-Feng Zou1, Xing-Fu Chen2, Karl Egil Malterud3, Frode Rise4, Hilde Barsett3, Kari Tvete Inngjerdingen3, Terje Einar Michaelsen3, Berit Smestad Paulsen3.
Abstract
Two pectic polysaccharides, 50 WCP-II-I and 100 WCP-II-I, were obtained from 50 and 100 °C water extracts of Codonopsis pilosula roots by ion exchange chromatography and gel filtration. The study of the sub-fractions obtained after pectinase degradation showed that the complement fixation activities of these pectins are expressed mainly by their ramified regions. The structure studies of native and sub-fractions showed the 50 WCP-II-I is a pectic polysaccharide, with long homogalacturonan regions (some of the galacturonic acid units were methyl esterified), interrupted by one short rhamnogalacturonan I (RG-I) region. The side chains of the RG-I region are arabinogalactan type I (AG-I) and type II (AG-II) attached on position 4 of rhamnose. The 100 WCP-II-I has two main ramified regions, one is galacturonan region with AG-I side chain on position 2 of GalA, and the other one is RG-I region with AG-II side chain on position 4 of Rha.Entities:
Keywords: Codonopsis pilosula; Complement fixation activity; Polysaccharides; Rhamnogalacturonan I
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Year: 2014 PMID： 25256503 DOI： 10.1016/j.carbpol.2014.07.036
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381