| Literature DB >> 25255155 |
Xiaolan Ye1, Di Cao1, Xin Zhao2, Fenyun Song3, Qinghua Huang3, Guorong Fan4, Fuhai Wu5.
Abstract
A method incorporating HPLC-PDA-IT-MS(n) with HPLC-Quadrupole-Orbitrap-MS was developed for the investigation of chemical fingerprint of Citrus reticulate 'Chachi' decoction (CRCD) and metabolic profile of SD rat plasma sample after oral administration of CRCD (1.5 g herb/kg). A total of 27 chemical constituents of CRCD were identified from their MW, UV spectra, MS(n) data and retention behavior by comparing the results with those of the reference standards or literature. And 43 compounds were detected in dosed SD rat plasma samples, including 9 prototypes which were identified as hesperetin, isosinensetin, sinensetin, tetramethyl-O-isoscutellarein, nobiletin, tetramethyl-O-scutellarein, HMF (3,5,6,7,8,3',4'-heptamethoxyflavone), tangeretin and 5-demethylnobiletin and 34 metabolites underwent metabolic process of demethylation, glucuronide conjugation, sulfate conjugation or mixed modes. This is the first research for the metabolic profile of CRCD in SD rats, which could lay a foundation for the further studies of CRC or its formulation.Entities:
Keywords: Chemical fingerprint; Citrus reticulate ‘Chachi’ decoction; HPLC-PDA-IT-MS(n); HPLC-Quadrupole-Orbitrap-MS; Metabolic profile
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Year: 2014 PMID： 25255155 DOI： 10.1016/j.jchromb.2014.06.035
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205