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Literature DB >> 25254149

Irises: A practical tool for image-based analysis of cellular DNA content.

Julia L Moore Vogel¹, David Michaelson², Anthony Santella¹, E Jane Albert Hubbard³, Zhirong Bao¹.

Abstract

The DNA content of nuclei is a valuable measure of cell cycle status. Irises is a software tool to facilitate systematic in situ determination of DNA content for cell cycle analysis at single-nucleus resolution within complex tissues. We demonstrate the utility of the tool with analysis of DNA content in germline nuclei of C. elegans. Compared with results obtained by manual analysis, we find the tool greatly facilitates analysis by improving speed at least 5-fold while maintaining accuracy. The source code and instruction manual (including installation for both Mac and PC) are provided.

Entities: Species

Keywords: C. elegans; cell cycle; germline; image analysis; ploidy

Year: 2014 PMID： 25254149 PMCID： PMC4165541 DOI： 10.4161/worm.29041

Source DB: PubMed Journal: Worm ISSN： 2162-4046

14 in total

1. Exploiting the dynamics of S-phase tracers in developing brain: interkinetic nuclear migration for cells entering versus leaving the S-phase.

Authors: N L Hayes; R S Nowakowski
Journal: Dev Neurosci Date: 2000 Impact factor: 2.984

Irises: A practical tool for image-based analysis of cellular DNA content.

1. Exploiting the dynamics of S-phase tracers in developing brain: interkinetic nuclear migration for cells entering versus leaving the S-phase.

Review 2. Regulating proliferation during retinal development.

3. Defining the epithelial stem cell niche in skin.

Review 4. Caenorhabditis elegans germ line: a model for stem cell biology.

5. Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline.

6. Axonal guidance mutants of Caenorhabditis elegans identified by filling sensory neurons with fluorescein dyes.

7. Insulin signaling promotes germline proliferation in C. elegans.

Review 8. Insulin and germline proliferation in Caenorhabditis elegans.

9. A hybrid blob-slice model for accurate and efficient detection of fluorescence labeled nuclei in 3D.

10. AceTree: a tool for visual analysis of Caenorhabditis elegans embryogenesis.

1. Cell cycle features of C. elegans germline stem/progenitor cells vary temporally and spatially.

2. Cell-cycle quiescence maintains Caenorhabditis elegans germline stem cells independent of GLP-1/Notch.