Muholan Kanapathy1, Nikhil M Patel1, Deepak M Kalaskar1, Afshin Mosahebi2, Babak J Mehrara3, Alexander M Seifalian4. 1. Division of Surgery & Interventional Science, UCL Centre for Nanotechnology & Regenerative Medicine, University College London, London, United Kingdom. 2. Division of Surgery & Interventional Science, UCL Centre for Nanotechnology & Regenerative Medicine, University College London, London, United Kingdom; Department of Plastic and Reconstructive Surgery, Royal Free London NHS Foundation Trust Hospital, London, United Kingdom. 3. Department of Plastic and Reconstructive Surgery, Memorial Sloan-Kettering Cancer Centre, New York. 4. Division of Surgery & Interventional Science, UCL Centre for Nanotechnology & Regenerative Medicine, University College London, London, United Kingdom. Electronic address: a.seifalian@ucl.ac.uk.
Abstract
BACKGROUND: Lymphedema is a chronic debilitating condition and curative treatment is yet to be found. Tissue engineering approach, which combines cellular components, scaffold, and molecular signals hold great potential in the treatment of secondary lymphedema with the advent of lymphatic graft to reconstruct damaged collecting lymphatic vessel. This review highlights the ideal characteristics of lymphatic graft, the limitation and challenges faced, and the approaches in developing tissue-engineered lymphatic graft. METHODS: Literature on tissue engineering of lymphatic system and lymphatic tissue biology was reviewed. RESULTS: The prime challenge in the design and manufacturing of this graft is producing endothelialized conduit with intraluminal valves. Suitable scaffold material is needed to ensure stability and functionality of the construct. Endothelialization of the construct can be enhanced via biofunctionalization and nanotopography, which mimics extracellular matrix. Nanocomposite polymers with improved performance over existing biomaterials are likely to benefit the development of lymphatic graft. CONCLUSIONS: With the in-depth understanding of tissue engineering, nanotechnology, and improved knowledge on the biology of lymphatic regeneration, the aspiration to develop successful lymphatic graft is well achievable.
BACKGROUND:Lymphedema is a chronic debilitating condition and curative treatment is yet to be found. Tissue engineering approach, which combines cellular components, scaffold, and molecular signals hold great potential in the treatment of secondary lymphedema with the advent of lymphatic graft to reconstruct damaged collecting lymphatic vessel. This review highlights the ideal characteristics of lymphatic graft, the limitation and challenges faced, and the approaches in developing tissue-engineered lymphatic graft. METHODS: Literature on tissue engineering of lymphatic system and lymphatic tissue biology was reviewed. RESULTS: The prime challenge in the design and manufacturing of this graft is producing endothelialized conduit with intraluminal valves. Suitable scaffold material is needed to ensure stability and functionality of the construct. Endothelialization of the construct can be enhanced via biofunctionalization and nanotopography, which mimics extracellular matrix. Nanocomposite polymers with improved performance over existing biomaterials are likely to benefit the development of lymphatic graft. CONCLUSIONS: With the in-depth understanding of tissue engineering, nanotechnology, and improved knowledge on the biology of lymphatic regeneration, the aspiration to develop successful lymphatic graft is well achievable.
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