A Lùrati1, A Laria2, D Mazzocchi3, K A Re4, M Marrazza5, M Scarpellini6. 1. Rheumatology Unit Fornaroli Hospital Magenta Italy Via Donatore Sangue 50, Milan 20013, Italy. Electronic address: alfredomaria.lurati@pec.it. 2. Rheumatology Unit Fornaroli Hospital Magenta Italy Via Donatore Sangue 50, Milan 20013, Italy. Electronic address: lariantonella@yahoo.it. 3. Rheumatology Unit Fornaroli Hospital Magenta Italy Via Donatore Sangue 50, Milan 20013, Italy. Electronic address: Daniela.mazzocchi@ao-legnano.it. 4. Rheumatology Unit Fornaroli Hospital Magenta Italy Via Donatore Sangue 50, Milan 20013, Italy. Electronic address: catia.re@ao-legnano.it. 5. Rheumatology Unit Fornaroli Hospital Magenta Italy Via Donatore Sangue 50, Milan 20013, Italy. Electronic address: mariagrazia.marrazza@ao-legnano.it. 6. Rheumatology Unit Fornaroli Hospital Magenta Italy Via Donatore Sangue 50, Milan 20013, Italy. Electronic address: magda.scarpellini@ao-legnano.it.
Abstract
OBJECTIVE: Determine Th lymphocytes concentration in patients with knee or hip osteoarthritis (OA). Evaluate their change after HA viscosupplementation. METHODS: Patients with early primary knee or hip OA (ACR Criteria) were recruited in two groups: group A was only observed longitudinally, group B was treated with a course of three weekly intra-articular injections of HA. A healthy control group gender and age matched was enrolled too. All subjects were followed for 3 months. Flow cytometry was performed from blood samples to assess T cells subpopulations (CD3, CD4, CD8, CCR6, CD38, CxCR3, HLA DR) at baseline and at 3-months visit. RESULTS: 86 patients were recruited with OA: 49 in Group A (35 knee OA, 14 hip OA), 37 in Group B (24 knee OA, 13 hip OA). 23 in Control Group. Activated CD4 T cells (CD4(+)CD38(+)DR(+), CD4(+)CD38(-)DR(+)), Th2 (CD4(+)CXCR3(-)CCR6(-)),Th1 (CD4(+)CXCR3(+)CCR6(-)) were higher at baseline in group A and B than in control group. After the HA course activated T cells were lower in group B than in group A (P = 0.01). Th17 (CD4(+)CXCR3(-)CCR6(+)) at baseline were higher in groups A and B than in control group and decreased levels in Group B after the HA course were observed (P = 0.03). CONCLUSION: The presence of activated T cells in patients with OA confirm that OA is a disease with an immunological/inflammatory involvement. Our preliminary results seems to show that HA injections could lower the levels of activated T cells, and so regulate the articular milieu.
OBJECTIVE: Determine Th lymphocytes concentration in patients with knee or hip osteoarthritis (OA). Evaluate their change after HA viscosupplementation. METHODS:Patients with early primary knee or hip OA (ACR Criteria) were recruited in two groups: group A was only observed longitudinally, group B was treated with a course of three weekly intra-articular injections of HA. A healthy control group gender and age matched was enrolled too. All subjects were followed for 3 months. Flow cytometry was performed from blood samples to assess T cells subpopulations (CD3, CD4, CD8, CCR6, CD38, CxCR3, HLA DR) at baseline and at 3-months visit. RESULTS: 86 patients were recruited with OA: 49 in Group A (35 knee OA, 14 hip OA), 37 in Group B (24 knee OA, 13 hip OA). 23 in Control Group. Activated CD4 T cells (CD4(+)CD38(+)DR(+), CD4(+)CD38(-)DR(+)), Th2 (CD4(+)CXCR3(-)CCR6(-)),Th1 (CD4(+)CXCR3(+)CCR6(-)) were higher at baseline in group A and B than in control group. After the HA course activated T cells were lower in group B than in group A (P = 0.01). Th17 (CD4(+)CXCR3(-)CCR6(+)) at baseline were higher in groups A and B than in control group and decreased levels in Group B after the HA course were observed (P = 0.03). CONCLUSION: The presence of activated T cells in patients with OA confirm that OA is a disease with an immunological/inflammatory involvement. Our preliminary results seems to show that HA injections could lower the levels of activated T cells, and so regulate the articular milieu.
Authors: Dawid Bączkowicz; Grzegorz Skiba; Mirosław Szmajda; Ivan Vařeka; Krzysztof Falkowski; Kevin Laudner Journal: Cartilage Date: 2019-05-09 Impact factor: 4.634