Literature DB >> 25245685

Using correlative light and electron microscopy to study zebrafish vascular morphogenesis.

Jacky G Goetz1, Fabien Monduc, Yannick Schwab, Julien Vermot.   

Abstract

Live imaging is extremely useful to characterize the dynamics of cellular events in vivo, yet it is limited in terms of spatial resolution. Correlative light and electron microscopy (CLEM) allows combining live confocal microscopy with electron microscopy (EM) for the characterization of biological samples at high temporal and spatial resolution. Here we describe a protocol allowing extracting endothelial cell ultrastructure after having imaged the same cell in its in vivo context through live confocal imaging during zebrafish embryonic development.

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Year:  2015        PMID： 25245685     DOI： 10.1007/978-1-4939-1164-6_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

Review 1.  Metastasis of circulating tumor cells: favorable soil or suitable biomechanics, or both?

Authors:  Ana Sofia Azevedo; Gautier Follain; Shankar Patthabhiraman; Sébastien Harlepp; Jacky G Goetz
Journal:  Cell Adh Migr       Date:  2015-08-27       Impact factor: 3.405

2.  Multicellular cuddling in a stem cell niche.

Authors:  Andrés Hidalgo; Jacky G Goetz
Journal:  Cell Adh Migr       Date:  2015-07-24       Impact factor: 3.405

Review 3.  One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.

Authors:  Xavier Heiligenstein; Miriam S Lucas
Journal:  Front Cell Dev Biol       Date:  2022-06-30

4.  Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson's Disease, Alzheimer's Disease and Type 2 Diabetes Mellitus.

Authors:  Greta M de Waal; Lize Engelbrecht; Tanja Davis; Willem J S de Villiers; Douglas B Kell; Etheresia Pretorius
Journal:  Sci Rep       Date:  2018-11-14       Impact factor: 4.379

5.  Correlative Light and Electron Microscopy of Rare Cell Populations in Zebrafish Embryos Using Laser Marks.

Authors:  Mohammad Goudarzi; Karina Mildner; Felix Babatz; Dietmar Riedel; Christian Klämbt; Dagmar Zeuschner; Erez Raz
Journal:  Zebrafish       Date:  2015-10-08       Impact factor: 1.985
  5 in total

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