Emmanouil Angelakis1, Oleg Mediannikov2, Cristina Socolovschi3, Nadjet Mouffok4, Hubert Bassene2, Adama Tall5, Hamidou Niangaly6, Ogobara Doumbo6, Abir Znazen7, Mhammed Sarih8, Cheikh Sokhna2, Didier Raoult2. 1. URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France. Electronic address: angelotasmanos@msn.com. 2. URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France; Campus Commun UCAD-IRD of Hann, BP 1386, CP 18524, Dakar, Senegal. 3. URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France. 4. Service des Maladies Infectieuses, Centre Hospitalo-Universitaire d'Oran, Oran, Algeria. 5. Institut Pasteur de Dakar, Dakar, Senegal. 6. Malaria Research and Training Centre, Faculty of Medicine, University of Sciences Techniques and Technology, Bamako, Bamako, Mali. 7. Laboratory of Microbiology and Laboratory of Research 'MPH', Habib Bourguiba University Hospital, Sfax, Tunisia. 8. Laboratoire des Maladies Vectorielles, Institut Pasteur du Maroc, Casablanca, Morocco.
Abstract
OBJECTIVES: Q fever has been reported throughout the African continent. The objective of this study was to detect the presence of Coxiella burnetii in febrile patients from Africa. METHODS: Blood samples from febrile and non-febrile patients from six African countries and from France were investigated retrospectively for Q fever infection by molecular assays targeting the IS1111 and IS30A spacers. RESULTS: We tested 1888 febrile patients from Senegal, Mali, Tunisia, Algeria, Gabon, and Morocco and found one male adult patient (0.3%) infected with C. burnetii in Algeria and six positive patients (0.5%) in Senegal. For one patient from Senegal we determined that the infection was caused by C. burnetii genotype 35. In Senegal, more patients were infected with C. burnetii in Keur Momar Sarr (p=0.002) than in the other locations. Blood samples taken from 500 (51% males) non-febrile people from Senegal and France were all negative. CONCLUSIONS: The installation of point-of-care laboratories in rural Africa can be a very effective tool for studying the epidemiology of many infectious diseases.
OBJECTIVES: Q fever has been reported throughout the African continent. The objective of this study was to detect the presence of Coxiella burnetii in febrile patients from Africa. METHODS: Blood samples from febrile and non-febrile patients from six African countries and from France were investigated retrospectively for Q fever infection by molecular assays targeting the IS1111 and IS30A spacers. RESULTS: We tested 1888 febrile patients from Senegal, Mali, Tunisia, Algeria, Gabon, and Morocco and found one male adult patient (0.3%) infected with C. burnetii in Algeria and six positive patients (0.5%) in Senegal. For one patient from Senegal we determined that the infection was caused by C. burnetii genotype 35. In Senegal, more patients were infected with C. burnetii in Keur Momar Sarr (p=0.002) than in the other locations. Blood samples taken from 500 (51% males) non-febrile people from Senegal and France were all negative. CONCLUSIONS: The installation of point-of-care laboratories in rural Africa can be a very effective tool for studying the epidemiology of many infectious diseases.
Authors: Gaël Mourembou; Florence Fenollar; Cristina Socolovschi; Guy Joseph Lemamy; Hermann Nzoughe; Lady Charlene Kouna; Fousseyni Toure-Ndouo; Matthieu Million; Angelique Ndjoyi Mbiguino; Jean Bernard Lekana-Douki; Didier Raoult Journal: Am J Trop Med Hyg Date: 2015-03-23 Impact factor: 2.345
Authors: Ndyetabura O Theonest; Ryan W Carter; Elizabeth Kasagama; Julius D Keyyu; Gabriel M Shirima; Rigobert Tarimo; Kate M Thomas; Nick Wheelhouse; Venance P Maro; Daniel T Haydon; Joram J Buza; Kathryn J Allan; Jo E B Halliday Journal: Vet Med Sci Date: 2020-12-05