Alteration of DNA binding activity of transcription factors in NRK-52E rat proximal tubular cells treated with cadmium.

Cadmium is a toxic heavy metal that causes severe clinical symptoms in various tissues including the kidney. In this study, activities of transcription factors were measured to identify what type of transcription factor was affected by cadmium in rat proximal tubular cells (NRK-52E cells) using the protein/DNA binding assay. After treatment of NRK-52E cells with 5 µM CdCl2 for 3 hr, nuclear extracts were used for the protein/DNA binding assay. Among 65 transcription factors, cadmium increased the activities of 6 transcription factors by more than 2.0-fold and decreased those of 15 transcription factors by less than 0.5-fold. These findings may provide new information about novel transcription factors associated with the mechanism of cadmium toxicity.