Literature DB >> 25241642

Freezing and storage at -20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis.

Laurence Delhaes1, Denis Filisetti2, Marie-Pierre Brenier-Pinchart3, Hervé Pelloux3, Hélène Yéra4, Frédéric Dalle5, Yvon Sterkers6, Emmanuelle Varlet-Marie7, Feriel Touafek8, Sophie Cassaing9, Patrick Bastien10.   

Abstract

Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR. No significant differences were found depending on duration or storage conditions at -20 °C or -80 °C.
Copyright © 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Amniotic fluid; Congenital toxoplasmosis; DNA storage; Real-Time PCR; Toxoplasma gondii

Mesh：

Substances：

Year:  2014        PMID： 25241642     DOI： 10.1016/j.diagmicrobio.2014.08.007

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  7 in total

1.  Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis.

Authors:  Denis Filisetti; Yvon Sterkers; Marie-Pierre Brenier-Pinchart; Sophie Cassaing; Frédéric Dalle; Laurence Delhaes; Hervé Pelloux; Fériel Touafek; Emmanuelle Varlet-Marie; Hélène Yera; Ermano Candolfi; Patrick Bastien
Journal:  J Clin Microbiol       Date:  2014-10-22       Impact factor: 5.948

2.  Evaluation of Toxoplasma ELITe MGB Real-Time PCR Assay for Diagnosis of Toxoplasmosis.

Authors:  Florence Robert-Gangneux; Marie-Pierre Brenier-Pinchart; Hélène Yera; Sorya Belaz; Emmanuelle Varlet-Marie; Patrick Bastien
Journal:  J Clin Microbiol       Date:  2017-02-15       Impact factor: 5.948

3.  DNA preservation in silk.

Authors:  Yawen Liu; Zhaozhu Zheng; He Gong; Meng Liu; Shaozhe Guo; Gang Li; Xiaoqin Wang; David L Kaplan
Journal:  Biomater Sci       Date:  2017-06-27       Impact factor: 6.843

4.  Direct genetic characterization of Toxoplasma gondii from clinical samples from Denmark: not only genotypes II and III.

Authors:  P Jokelainen; J-B Murat; H V Nielsen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-12-03       Impact factor: 3.267

5.  Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.

Authors:  Guillaume Roux; Christophe Ravel; Emmanuelle Varlet-Marie; Rachel Jendrowiak; Patrick Bastien; Yvon Sterkers
Journal:  PLoS One       Date:  2019-09-27       Impact factor: 3.240

6.  Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples.

Authors:  Marie-Pierre Brenier-Pinchart; Emmanuelle Varlet-Marie; Florence Robert-Gangneux; Denis Filisetti; Juliette Guitard; Yvon Sterkers; Hélène Yera; Hervé Pelloux; Patrick Bastien
Journal:  PLoS One       Date:  2021-02-17       Impact factor: 3.240

7.  Anatomical distribution of Toxoplasma gondii in naturally and experimentally infected lambs.

Authors:  Myriam Thomas; Dominique Aubert; Sandie Escotte-Binet; Benoît Durand; Céline Robert; Régine Geers; Annie Alliot; Guillaume Belbis; Isabelle Villena; Radu Blaga
Journal:  Parasite       Date:  2022-02-01       Impact factor: 3.000
  7 in total

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