Pamela Yango1, Eran Altman2, James F Smith3, Peter C Klatsky4, Nam D Tran5. 1. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California. 2. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel. 3. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California; Department of Urology, University of California, San Francisco, San Francisco, California. 4. Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York. 5. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, California. Electronic address: trann@obgyn.ucsf.edu.
Abstract
OBJECTIVE: To determine whether optimal human spermatogonial stem cell (SSC) cryopreservation is best achieved with testicular tissue or single cell suspension cryopreservation. This study compares the effectiveness between these two approaches by using testicular SSEA-4+ cells, a known population containing SSCs. DESIGN: In vitro human testicular tissues. SETTING: Academic research unit. PATIENT(S): Adult testicular tissues (n=4) collected from subjects with normal spermatogenesis and normal fetal testicular tissues (n=3). INTERVENTION(S): Testicular tissue versus single cell suspension cryopreservation. MAIN OUTCOME MEASURE(S): Cell viability, total cell recovery per milligram of tissue, as well as viable and SSEA-4+ cell recovery. RESULT(S): Single cell suspension cryopreservation yielded higher recovery of SSEA-4+ cells enriched in adult SSCs, whereas fetal SSEA-4+ cell recovery was similar between testicular tissue and single cell suspension cryopreservation. CONCLUSION(S): Adult and fetal human SSEA-4+ populations exhibited differential sensitivity to cryopreservation based on whether they were cryopreserved in situ as testicular tissues or as single cells. Thus, optimal preservation of human SSCs depends on the patient's age, type of samples cryopreserved, and end points of therapeutic applications.
OBJECTIVE: To determine whether optimal human spermatogonial stem cell (SSC) cryopreservation is best achieved with testicular tissue or single cell suspension cryopreservation. This study compares the effectiveness between these two approaches by using testicular SSEA-4+ cells, a known population containing SSCs. DESIGN: In vitro human testicular tissues. SETTING: Academic research unit. PATIENT(S): Adult testicular tissues (n=4) collected from subjects with normal spermatogenesis and normal fetal testicular tissues (n=3). INTERVENTION(S): Testicular tissue versus single cell suspension cryopreservation. MAIN OUTCOME MEASURE(S): Cell viability, total cell recovery per milligram of tissue, as well as viable and SSEA-4+ cell recovery. RESULT(S): Single cell suspension cryopreservation yielded higher recovery of SSEA-4+ cells enriched in adult SSCs, whereas fetal SSEA-4+ cell recovery was similar between testicular tissue and single cell suspension cryopreservation. CONCLUSION(S): Adult and fetal human SSEA-4+ populations exhibited differential sensitivity to cryopreservation based on whether they were cryopreserved in situ as testicular tissues or as single cells. Thus, optimal preservation of human SSCs depends on the patient's age, type of samples cryopreserved, and end points of therapeutic applications.
Authors: Christine Wyns; Anne Van Langendonckt; François-Xavier Wese; Jacques Donnez; Mara Curaba Journal: Hum Reprod Date: 2008-07-28 Impact factor: 6.918
Authors: Eran Altman; Pamela Yango; Radwa Moustafa; James F Smith; Peter C Klatsky; Nam D Tran Journal: Reproduction Date: 2014-07-16 Impact factor: 3.906
Authors: Larissa Benvenutti; Rafael Alonso Salvador; David Til; Alfred Paul Senn; David Rivero Tames; Nicole Louise Lângaro Amaral; Vera Lúcia Lângaro Amaral Journal: JBRA Assist Reprod Date: 2018-09-01